RNA-seq workflow: gene-level exploratory analysis and differential expression
Here we walk through an end-to-end gene-level RNA-seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were quantified to the reference transcripts, and prepare gene-level count datasets for downstream analysis. We will perform explo...Tags: RNA-seq, workflow, gene-level, exploratory, analysis, differential, expression
942 days ago
Tools for Differential expression analysis
apeglm - https://bioconductor.org/packages/release/bioc/html/apeglm.html ashr - https://github.com/stephens999/ashr, https://cran.r-project.org/web/packages/ashr/index.html consensusDE - https://bioconductor.org/packages/release/bioc/html/consensusDE.html DES...Tags: Differential, expression, analysis, Tools
547 days ago