Meraculous: De Novo Genome Assembly with Short Paired-End Reads
We describe a new algorithm, meraculous, for whole genome assembly of deep paired-end short reads, and apply it to the assembly of a dataset of paired 75-bp Illumina reads derived from the 15.4 megaba...or correction step as used in other short-read...2387 days ago
QuorUM: An Error Corrector for Illumina Reads
Illumina Sequencing data can provide high coverage of a genome b...(most often 100 bp to 150 bp) reads at a low cost. Even with low...average has an error in some read at every base in the genome....erroneous k-mers in the reads. However, there is enough in...at error correcting Illumina reads...2385 days ago
Unicycler: Hybrid assembly pipeline for bacterial genomes
Unicycler is an assembly pipeline for bacterial genomes. It can assemble Illumina-only read sets where it functions as a SPAdes-optimiser. It can also assembly long-read-only sets (PacBio or&nbs...ossible assemblies, give it both Illumina reads...2384 days ago
2374 days ago
TULIP - The Uncorrected Long read Itegration Pipeline
#Running TULIP (The Uncorrected Long-read Integration Process), version 0.4 late 2016 (Europ...;Tulipseed takes as input alignments files of long reads to sparse short seeds, and o...graph and scaffold structures. Tulipbulb adds long read...2371 days ago
miniasm: very fast OLC-based de novo assembler for noisy long reads
Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by&n...simply concatenates pieces of read sequences to generate the fin...ishes the assembly, including reads overlapping, in ~10 minutes...ontigs from raw PacBio or ONT reads...2367 days ago
Opera: An optimal genome scaffolding program
Opera (Optimal Paired-End Read Assembler) is a sequence assembly program (http://en.wi...nbsp;). It uses information from paired-end or long reads to optimally order and orient contigs assembled from shotgun-sequencing reads...2367 days ago
Oxford Nanopore Sequencing, Hybrid Error Correction, and de novo Assembly of a Eukaryotic Genome
Monitoring the progress of DNA molecules through a membrane pore has been...corr) specifically for Oxford Nanopore reads, as existing packages were incapable of assembling the long read lengths (5-50kbp) at such hig...e, the assembly with the long nanopore reads...2365 days ago
2365 days ago
Musket: a multistage k-mer spectrum based corrector
Musket is a well-established leading next-generation sequencing read error correction algorithm targetting Illumina sequencing. This corre...g substitution-error-based correctors. In addition, Musket is multi-threade...2358 days ago