Single molecule Sequencing (SMS)
3rd generation sequencers
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Single molecule Sequencing (SMS)
Owner: Rahul Agarwal
Group members: 4
Description:
SMS is now slowly gaining place in sequencing world, more and more scientists now keen to use SMS method to sequence molecules in comparatively less complex protocal and less time. One of most successful so far is SMRT from Pacbio and others will be flood the market soon and may be one day it replaces 2nd generation sequencer.
This group is meant to share articles, development and application arise from arrival of SMS method.
for instance,
Brief description: 3rd generation sequencers
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Resolving the complexity of the human genome using single-molecule sequencing
By Rahul Agarwal 3212 days agowww.nature.com - The human genome is arguably the most complete mammalian reference assembly yet more than 160 euchromatic gaps remain and aspects of its structural variation remain poorly understood ten years after its completion. The results in this paper...Assembly and diploid architecture of an individual human genome via single-molecule technologies
By Rahul Agarwal 3212 days agowww.nature.com - The research work shows that it is now possible to integrate single-molecule and high-throughput sequence data to generate de novo assembled genomes that approach reference...Scaffolding of a bacterial genome using MinION nanopore sequencing
By Rahul Agarwal 3216 days agowww.nature.com - Second generation sequencing has revolutionized genomic studies. However, most genomes contain repeated DNA elements that are longer than the read lengths achievable with typical sequencers, so the genomic order of several generated contigs cannot...GraphMap - A highly sensitive and accurate mapper for long, error-prone reads
By Rahul Agarwal 3217 days ago Comments (1)github.com - GraphMap is a novel mapper targeted at aligning long, error-prone third-generation sequencing data.It is designed to handle Oxford Nanopore MinION 1d and 2d reads with very high sensitivity and accuracy, and also presents a significant...
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- Jit commented on a bookmark GraphMap - A highly sensitive and accurate mapper for long, error-prone reads
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