A major breakthrough (replaced microarrays) in the late 00’s and has been widely used since
Measures the average expression level for each gene across a large population of input cells
Useful for comparative transcriptomics,...
When you have both Illumina and Nanopore data, then SPAdes remains a good option for hybrid assembly - SPAdes was used to produce the B fragilis assembly by Mick Watson’s group.
Again, running spades.py will show you the...
kallisto
Transcript abundance quantification from RNA-seq data (uses pseudoalignment for rapid determination of read compatibility with targets)
Software (C++)
https://pachterlab.github.io/kallisto/
Sailfish
Estimation of isoform abundances...
analog -- A feature that appears similar in two taxa which have originated from two different ancestors.
ancestor -- Any organism, population, or species from which some other organism, population, or species is descended by...
It is often necessary to import sample textbook data into R before you start working on your homework.
Excel File
Quite frequently, the sample data is in Excel format, and needs to be imported into R prior to use. For this, we can...
A curated list of awesome Perl frameworks, libraries and software.
major/MySQLTuner-perl - MySQLTuner is a script written in Perl that will assist you with your MySQL configuration and make recommendations for increased performance and...
fragment size: the Illumina WGS protocol generates paired-end reads from both ends of longer fragments. The lengths of these fragments are assumed to be sampled from a normal distribution. Therefore, in the absence of structural variants,...
An algorithm is a procedure for solving a problem in terms of the actions to be executed and the order in which those actions are to be executed. An algorithm is merely the sequence of steps taken to solve a problem. The steps are normally...
The genome assemblers generally take a file of short sequence reads and a file of quality-value as the input. Since the quality-value file for the high throughput short reads is usually highly memory-intensive, only a few assemblers, best suited for...