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Question: Question: How to validate the assembly?

Shruti Paniwala
3032 days ago

Question: How to validate the assembly?

I am working on assembly of new genome. I am just wondering how to validate my assembled genome? 

Answers
0

There are varied sources of information that can provide clues regarding the correctness of assemblies. Examples of such data are: Mate-pair information, unused read information, correlated polymorphisms, consistency of assembly with the parameters of the sequencing process and optical mapping data. 

Following tools are useful for validation process:

0

You can try the following as well:

Assembly Likelihood Evaluation (ALE) framework http://bioinformatics.oxfordjournals.org/content/29/4/435

Computing Genome Assembly Likelihoods (CGAL) http://genomebiology.biomedcentral.com/articles/10.1186/gb-2013-14-1-r8

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I used these two tools for validaions, hope useful. 

REAPR: a universal tool for genome assembly evaluation http://genomebiology.biomedcentral.com/articles/10.1186/gb-2013-14-5-r47

QUAST: quality assessment tool for genome assemblies http://bioinformatics.oxfordjournals.org/content/29/8/1072