Rahul Nayak2188 days ago
I recommend to have a look at this BOL
For De-novo Genome Assembly -- Canu. Flye and MARVEL sound promising.Alignment -- minimap2 and/or LAST, depending on the application.
LAST is particularly good at quickly picking up duplicated adapters in chimeric reads. Check out this script https://github.com/gringer/bioinfscripts/blob/master/maf_bcsplit.pl for parsing.
Please visit this https://bioinformaticsonline.com/search?q=ont&entity_type=object&entity_subtype=bookmarks&offset=0&search_type=entities
I recommend to have a look at this BOL
For De-novo Genome Assembly -- Canu. Flye and MARVEL sound promising.
Alignment -- minimap2 and/or LAST, depending on the application.
LAST is particularly good at quickly picking up duplicated adapters in chimeric reads. Check out this script https://github.com/gringer/bioinfscripts/blob/master/maf_bcsplit.pl for parsing.