Trying to understand HiFi reads, any link could help ...
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By calling consensus from subbreads produced by multiple passes of the enzyme around a circularized template, HiFi reads are generated. This results in both lengthy and effective HiFi reads. In comparison, traditional PacBio long reads are created by a single pass of the enzyme around a circularized template, which does not provide the high accuracy feature of HiFi reads, although it allows for longer read lengths overall.
By calling consensus from subbreads produced by multiple passes of the enzyme around a circularized template, HiFi reads are generated. This results in both lengthy and effective HiFi reads. In comparison, traditional PacBio long reads are created by a single pass of the enzyme around a circularized template, which does not provide the high accuracy feature of HiFi reads, although it allows for longer read lengths overall.
Find out more about how SMRT Sequencing runs at https://www.pacb.com/smrt-science/smrt-sequencing/hifi-reads-for-highly-accurate-long-read-sequencing/