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Hi Andaleeb,
You can follow methods used in this paper "Reconstruction and In Silico Analysis of Metabolic Network for an Oleaginous Yeast, Yarrowia lipolytica" http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051535
Thanks
Hi Andaleeb,
Please go through this paper http://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.1003126 which explain the reconstruction and validation of a genome-scale metabolic model for the neurospora crassa using Fast Automated Reconstruction of Metabolism (FARM). Similarly, In silico genome-scale reconstruction and validation of the Staphylococcus aureus metabolic network was discussed in http://www.ncbi.nlm.nih.gov/pubmed/16155945.
Cheers