This is a tutorial for a workshop on long-read (PacBio) genome assembly.
It demonstrates how to use long PacBio sequencing reads to assemble a bacterial genome, and includes additional steps for circularising, trimming, finding plasmids, and correcting the assembly with short-read Illumina data.
Please comment if you know any other long read addembly tutorial.
Comments
Long reads assembler https://zenodo.org/record/345098/files/scientific_reports_assembly_long_reads%282%29.pdf
Long reads assembly PPT https://www.msi.umn.edu/sites/default/files/Badalamenti_PacBio_tutorial_12-10-2014.pdf
Bacterial genome assembly tutorial http://evomics.org/learning/assembly-and-alignment/2017-assembly-lab/
Long reads base bacterial genome assembly https://inf-biox121.readthedocs.io/en/2016/Assembly/practicals/07_Assembly_using_minasm+racon.html