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  • mosdepth: fast BAM/CRAM depth calculation for WGS, exome, or targeted sequencing

mosdepth: fast BAM/CRAM depth calculation for WGS, exome, or targeted sequencing

https://github.com/brentp/mosdepth

mosdepth can output:

per-base depth about 2x as fast samtools depth--about 25 minutes of CPU time for a 30X genome.
mean per-window depth given a window size--as would be used for CNV calling.
the mean per-region given a BED file of regions.
a distribution of proportion of bases covered at or above a given threshold for each chromosome and genome-wide.
quantized output that merges adjacent bases as long as they fall in the same coverage bins e.g. (10-20)
threshold output to indicate how many bases in each region are covered at the given thresholds.
A summary of mean depths per chromosome and within specified regions per chromosome.