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  • Supernova: generates phased, whole-genome de novo assemblies from a Chromium-prepared library.

Supernova: generates phased, whole-genome de novo assemblies from a Chromium-prepared library.

https://support.10xgenomics.com/de-novo-assembly/software/pipelines/latest/using/running

Supernova generates phased, whole-genome de novo assemblies from a Chromium-prepared library.

Please see Achieving Success with De Novo Assembly and System Requirements before creating your Chromium libraries for assembly.

Supernova should be run using 38-56x coverage of the genome.
• Somewhat higher coverage is sometimes advantageous.
• Supernova will exit if it finds that coverage is far from the recommended range.
• Note that at most 2.14 billion reads are allowed.
• Please note that we have not extensively tested genomes larger than human, and any genome above approximately 4 GB should be considered experimental and is not supported.