BOL: Is reference genome necessary for gene expression study in transcriptome sequencing or for variant discovery in genome sequencing?

Discussion
Next Generation Sequencing (NGS)
Is reference genome necessary for gene expression study in transcriptome sequencing or for variant...

Is reference genome necessary for gene expression study in transcriptome sequencing or for variant discovery in genome sequencing?

Is reference genome necessary for gene expression study in transcriptome sequencing or for variant...

Started by Rahul Agarwal 4175 days ago

Sequencing
RNA
DNA
Genome

Like in case of plant genomes where nature of genome is too complex and huge in size to accomplish complete de novo assembly by current sequencing technology. What would be alternate solution? Can we live in reference free world?

BOL

Next Generation Sequencing (NGS)

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Is reference genome necessary for gene expression study in transcriptome sequencing or for variant discovery in genome sequencing?

Is reference genome necessary for gene expression study in transcriptome sequencing or for variant...