Next Generation Sequencing (NGS)
https://en.wikipedia.org/wiki/Sequencing
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Next Generation Sequencing (NGS)
Owner: Rahul Agarwal
Group members: 38
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This group meant to discuss different sequencing methods and their data analysis. Now, sequencing is almost one of the most popular technique scientific fraternity pursue worldwide to comprehand the genomic complexity of all the organism and also used to reveal genetic factors responsible for different traits or disease
Brief description: https://en.wikipedia.org/wiki/Sequencing
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World of Omics
By Rahul Agarwal 4146 days ago Comments (5)How many variants of "omics" techniques presently in use ?
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Alignment of closely related whole genomes/scaffolds
By Rahul Nayak 3219 days ago Comments (1)www.cs.utoronto.ca - With the relative ease and low cost of current generation sequencing technologies has led to a dramatic increase in the number of sequenced genomes for species across the tree of life. This increasing volume of data requires tools that can quickly...BioScripts
By Rahul Nayak 3434 days agocode.google.com - You are requested to please bookmark collection of bioinformatics tools, scripts, codes that can be pieced together in a very easy and flexible manner to perform both simple and complex bioinformatics tasks. The next-generation sequencing included...Orione – a web-based framework for NGS analysis in microbiology
By Martin Jones 3774 days agohttp://orione.crs4.it/ - End-to-end NGS microbiology data analysis requires a diversity of tools covering bacterial resequencing, de novo assembly, scaffolding, bacterial RNA-Seq, gene annotation and metagenomics. However, the construction of computational pipelines that...Integrative Genomics Viewer (IGV) tutorial
By Neel 3785 days agowikis.utexas.edu - The Integrative Genomics Viewer (IGV) from the Broad Center allows you to view several types of data files involved in any NGS analysis that employs a reference genome, including how reads from a dataset are mapped, gene annotations, and predicted...Next generation sequencing(NGS) books
By Abhimanyu Singh 3828 days ago Comments (1)en.wikibooks.org - Employing different technologies, the purpose of NGS platform is to decode the identity or modification on the nucleotides. NGS platforms evolve quickly and capture the main stream. This bookmark is created to provide NGS online books links.New RNA Seq tool
By Rahul Agarwal 3863 days ago Comments (4)www.genengnews.com - "By removing the time-consuming step of read mapping, the authors reported, Sailfish able to provide quantification estimates 20–30 times faster than current methods without loss of accuracy." Tool...
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- LEGE commented on a bookmark Next Generation Sequencing (NGS) Tutorials
- Rahul Nayak replied on the discussion topic Want to Know which genome assembler rule the world ?
- BioStar replied on the discussion topic Want to Know which genome assembler rule the world ?
- Priya Singh replied on the discussion topic Does anyone have Nanopore latest updates?
Group discussion
Does anyone have Nanopore latest updates?
Started by Poonam Mahapatra 4119 days ago Replies (34) Last reply by Priya Singh 2752 days agoThere was a lot of buzz about Oxford Nanopore Technologies® is developing the GridION™ system and miniaturised MinION™ device. These are a new generation of electronic molecular analysis system for use in scientific research,...Want to Know which genome assembler rule the world ?
Started by Rahul Agarwal 4120 days ago Replies (3) Last reply by Rahul Nayak 2150 days agoAssemblathon 2: evaluating de novo methods of genome...Compressive Genomics
Started by Rahul Agarwal 4120 days agoThe key to finding a solution is to notice that most genomicsequences differ by very little. It may well be that the number of complete genome sequences being stored is increasing rapidly, but the actual amount of new data is very small. In...Is reference genome necessary for gene expression study in transcriptome sequencing or for variant...
Started by Rahul Agarwal 4145 days agoLike in case of plant genomes where nature of genome is too complex and huge in size to accomplish complete de novo assembly by current sequencing technology. What would be alternate solution? Can we live in reference free world?
Group pages
Software packages for next gen sequence analysis
Last updated 3442 days ago by Rahul Nayak Comments (5)Integrated solutions * CLCbio Genomics Workbench - de novo and reference assembly of Sanger, Roche FLX, Illumina, Helicos, and SOLiD data. Commercial next-gen-seq software that extends the CLCbio Main Workbench software. Includes SNP detection,...-
Commercial and public next-gen-seq (NGS) software
Last updated 3719 days ago by Partek Comments (7)Integrated solutions CLCbio Genomics Workbench - de novo and reference assembly of Sanger, Roche FLX, Illumina, Helicos, and SOLiD data. Commercial next-gen-seq software that extends the CLCbio Main Workbench software. Includes SNP detection,... -
Useful Publications and Websites for Deep Sequencing Data Analysis
Last updated 3979 days ago by Rahul NayakGlobal overview papers Next generation quantitative genetics in plants. Jiménez-Gómez, Frontiers in Plant Science 2:77, 2011 Full Text [equally relevant to animal and microbial systems] Sense from sequence reads: methods for... -
Genome Browsers
Last updated 4114 days ago by Rahul Agarwal Comments (2)Genome Browser is the platform/database used for searching and retreiving sequences and annotation of genomes belong to various eukaryotes, prokaryotes, etc. Following are the weblink for different available... -
Structure of Binary files used for storing sequencing data-bam and sff
Last updated 4120 days ago by Rahul AgarwalMany times bioinformatician needs to parse binary files like bam and sff. Advantage of binary files is that they occupy less space in memory with maximum information content. Link for those who looking for structure of Bam and sff...
Group news
Sequencing By Xpansion
By Jitendra Narayan 3444 days agoXpandomer in a single drop reaction to sequence an entire human genome 1000X over.deepTools
By Martin Jones 3666 days agoUser-friendly tools for the normalization and visualization of deep-sequencing dataDrawback of Exome Sequencing
By Rahul Agarwal 3825 days agoSmaller Exome datasets were found to be worse performer than those from larger datasets (WGS)The genome factory !!!
By Madhvan Reddy 3962 days agoWorld’s first $1,000 genome enables ‘factory’ scale sequencing for population and disease studies
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