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deepTools
By Martin Jones 3715 days agoUser-friendly tools for the normalization and visualization of deep-sequencing dataStringTie Transcript assembly and quantification for RNA-Seq
By Jit 1676 days agoccb.jhu.edu - StringTie is a fast and highly efficient assembler of RNA-Seq alignments into potential transcripts. It uses a novel network flow algorithm as well as an optional de novo assembly step to assemble and quantitate full-length...Understanding RNA-Seq Normalization Methods: TPM vs. FPKM vs. CPM
By Neel 30 days agoChoosing the right normalization method depends on the specific objectives of your RNA-Seq analysis. TPM’s proportionality and robustness make it the preferred choice for most applications, while CPM serves well for differential expression...Webinar on Streamlining large scale analysis using the Strand NGS Pipeline Manager on 24 Feb 2016
By Yeshodari 3262 days agoLive Webinar on Streamlining large scale NGS data analysis using the Strand NGS Pipeline Manager on 24 Feb 2016 Abstract: Strand NGS includes comprehensive workflows for DNA-Seq, RNA-Seq, Small RNA-Seq, ChIP-Seq, MeDIP-Seq, and Methyl-Seq analysis....Webinar: Unravelling complex mutational events in clinical cases using the power of NGS data...
By Strand 2572 days agoNGS webinarVariant Calling Pipeline
By LEGE 82 days agogithub.com - The variantcalling.nf nextflow script will take any number of samples with paired-end reads in FASTQ format, map reads using Bowtie2, process BAM files, and finally call variants using BCFtools v1.21 and/or Freebayes v1.3.6. If part of the...Webinar on 'An integrated RNA and DNA approach to unravel genetic regulation in cancer'
By Yeshodari 3621 days agoWebinar, next generation sequencing data analysisRNA-seq Analysis Workshop Course Materials
By Jit 2383 days agochagall.med.cornell.edu - RNAseq can be roughly divided into two "types": Reference genome-based - an assembled genome exists for a species for which an RNAseq experiment is performed. It allows reads to be aligned against the reference genome and significantly improves...Modular, efficient and constant-memory single-cell RNA-seq preprocessing
By Jit 1376 days ago Comments (1)pachterlab.github.io - With kallisto | bustools you can Generate a cell x gene or cell x transcript equivalence class count matrix Perform RNA velocity and single-nuclei RNA-seq analsis Quantify data from numerous technologies such as...New RNA Seq tool
By Rahul Agarwal 3913 days ago Comments (4)www.genengnews.com - "By removing the time-consuming step of read mapping, the authors reported, Sailfish able to provide quantification estimates 20–30 times faster than current methods without loss of accuracy." Tool...
