Recovery of complete genomes from metagenomes
This project contains scripts and tutorials on how to assemble individual microbial genomes from metagenomes, as described in: Genome sequences of rare, uncultured bacteria obtained by differential coverage binning of multiple metagenomes Mads Albertsen, Philip Hugenholtz, Adam Skarsh...Tags: Recovery, complete, genomes, metagenomes
2349 days ago
HGT-Finder: A New Tool for Horizontal Gene Transfer Finding and Application to Aspergillus genomes
HGT-Finder: (i) can be used for HGT detection in both prokaryotes and eukaryotes, (ii) can report a statistical P value for each gene to indicate how likely it is to be horizontally transferred, and (iii) is fully automated (requires minimal human intervention), as well as very ea...Tags: HGT-Finder, Tool, Horizontal, Gene, Transfer, Finding, Application, Aspergillus, genomes
2328 days ago
Tags: Tallymer, method, compute, K-mer, frequencies, application, annotate, large, repetitive, plant, genomes
2299 days ago
Digest: In silico restriction digest of complete genomes
This tool allows to retrieve number of cleavages yielded by commercially available endonucleases in up-to-date sequenced prokaryotic genomes. When the number of fragments is bellow 50, Pulse Field gel Electrophoresis (PFGE) is simulated. A tool for restriction digest of longuser's sequences...Tags: Digest, In silico, restriction, digest, complete, genomes
2211 days ago
Tags: CheckM, Assessing, quality, microbial, genomes, recovered, isolates, single, cells, metagenomes, Validation
2202 days ago
GenomeMapper: Simultaneous alignment of short reads against multiple genomes
GenomeMapper is a short read mapping tool designed for accurate read alignments. It quickly aligns millions of reads either with ungapped or gapped alignments. It can be used to align against multiple genomes simulanteously or against a single reference. If you are unsure which one is the appropr...Tags: GenomeMapper, Simultaneous, alignment, short, reads, multiple, genomes
2200 days ago
Ranbow: a haplotype assembler for polyploid genomes
Ranbow is a haplotype assembler for polyploid genomes. It has been developed for the haplotype assembly of the hexaploid sweet potato genome, which is highly heterozygous. Ranbow can also be applied to other polyploid genomes. After a first phasing, Ranbow utilizes the assembled haplotypes to imp...Tags: Ranbow, haplotype, assembler, polyploid, genomes, Assembly, NGS
2193 days ago
mmgenome: Tools for extracting individual genomes from metagneomes
The mmgenome toolbox enables reproducible extraction of individual genomes from metagenomes. It builds on the multi-metagenome concept, but wraps most of the process of extracting genomes in simple R functions. Thereby making the whole process of binning easy and at the same time reprod...Tags: mmgenome, Tools, extracting, individual, genomes, metagneomes
2124 days ago
Tags: GTDB-Tk, toolkit, assign, objective, taxonomic, classifications, bacterial, archaeal, genomes
2111 days ago
BASE: a practical de novo assembler for large genomes using long NGS reads
new de novo assembler called BASE. It enhances the classic seed-extension approach by indexing the reads efficiently to generate adaptive seeds that have high probability to appear uniquely in the genome. Such seeds form the basis for BASE to build extension trees and then to use revers...Tags: BASE, practical, de novo, assembler, large, genomes, long, NGS, reads
2053 days ago