Ancestral sequence reconstruction steps !
...tral sequence reconstruction (ASR) – also known as ancestral gene/sequence reconstruction/resurrection – is a technique used...namics of protein evolution. BEAST is the best...ave as ...) Ideally do a tree-based groups. 4.3- RDP...2237 days ago
Gap filling or Contigs extensions tools !
...help to perform local re-assemblies using 454 reads. We used...CE software from the DeRisi lab. Its meant to do something ve...the IMAGE tool: http://genomebiology.com/2010/11/4/R41 A...ads. c. Construct a new assembly C' from C and the new reads...2223 days ago
LINKS scaffolder bloomfilter setting !
➜ bin git:(master) ✗ ls -l total 68 drwxrwxr-x 3 urbe urbe 4096 Jun 15 12:15 lib -rwx...-c++ -perl5 BloomFilter.i ➜ bloomfilter git:(master) ✗ g++...:(master) ✗ g++ -Wall -shared BloomFilter_wrap.o -o BloomFilt....1, optional) -l minimum number of links (k-mer pairs) to c...2209 days ago
Understanding BLASTn output format 6 !
BLASTn output format 6 BLASTn maps DNA against DNA, fo...ces against a reference genomeblastn -query genes.ffn -subject genome.fna -outfmt 6 BLASTn tabular output format 6...rt Start of alignment in subjectsend End of alignment...2196 days ago
Interview Puzzles for Bioinformatician !
...of Top 25 puzzles which have been asked in top Tech Intervie...ates and Gold Coin puzzle Probability of having boy Random...Gold Bar Puzzle Crossing the Bridge Puzzle Will you accept...uzzles-Top 25 Tech Interview Logical PuzzlesEach of the...2177 days ago
My commonly used commands in Bioinformatics
...evaluate them individually: minimap -t 24 assembly.fasta long_reads.fastq.gz | racon -t 24 long_reads.fastq.gz - assembly.fasta racon_assembly.fasta...mer.delta This reports Racon's changes in a table. You can exclude indels wit...2168 days ago
List of non-commercial NGS genotype-calling software
...developed statistical methods both improve and quantify the c...calling, and will especially benefit the growing number of s...pe caller (SOAPsnp) realSFS http://128.32.118.212/thorf...ration) and SNP quality recalibration (Variant Recalibrator)...2154 days ago
Parallel Processing with Perl !
...small tutorial on how to make best use of multiple processors for bioinformatics analysis. One be...this tutorial. Many times in bioinformatics we need to deal...te processor). Thats it!! One big disadvantage of forking is...aling with multiple processes because it will decrease the pr...2138 days ago
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