Command line to download blast database / protein
...abases are available ftp://ftp.ncbi.nlm.nih.gov/blast/db/ https://ftp.ncbi.nlm.nih.gov/blast/db/ # Database detail / description nr.*tar.gz | Non-redundant protein sequences...1002 days ago
Download desire version of Blast software !
...acking blast tar -zxvf ncbi-blast-2.6.0+-x64-linux.tar.gz #Slurm template #!/bin/bash #SBATCH --partition=longjobs #SBATCH --nodes=1 #SBATCH --ntasks-per-node=32 #SBATCH --time=1:00:00...1001 days ago
Downloading mmseqs databases !
...Type Taxonomy Url - UniRef100 Aminoacid yes https://www.uni...iref - UniRef50 Aminoacid yes https://www.uni...otkb - UniProtKB/Swiss-Prot Aminoacid yes https://uniprot.../a-new-pfam-b-is-released - eggNOG Profile...1001 days ago
987 days ago
Installing Covid19 Environment !
(base) vikas@vikas-Lenovo-ideapad-320-15ISK:~/vinodLab/Genepi$ conda env create -f covid19-envir...d. You still need to manually update the taxonomy databases before Krona can...c reports. The update script is ktUpdateTaxonomy.sh. The default location f...982 days ago
Bash script to simulate a genome !
...GCA_003401745.1_ASM340174v1_genomic.fna.gz | \ bgzip -@75 > G...GCA_003401745.1_ASM340174v1_genomic.fna_upper.diploid.fasta.gz...GCA_003401745.1_ASM340174v1_genomic.fna_upper.diploid.fasta.gz...GCA_003401745.1_ASM340174v1_genomic.fna_upper.diploid.fasta.gz...977 days ago
954 days ago
Extract fasta sequences with ids in another file !
#Ids are in test.txt - one ids per line #sequences are in test.fa grep -w -A 2 -f test.txt test.fa --no-group-separator # seqtk seqtk subseq test.fa test.txt #faSomeRecods faSomeRecords in.fa listFile out.fa # seqkit seqkit grep -n -f list.txt sequences.fas > newfile2.fas948 days ago
921 days ago
Extract the sequences with IDs !
#sed -i 's/\_/ /g' Delta_seqID_from_lineage_report.txt seqtk subseq genomic.fna Delta_seqID_from_lineage_report.txt > Delta.fasta #Split the fasta in 11 equal sequences subsets pyfasta split -n 11 Delta.fasta910 days ago