BBTools for bioinformatician !
...BMap by adding the flags "minid=0.76 maxindel=16k minhits=1". With those parameters it is extremely sensitive.NOTE: 2 BBSplit has different ambiguity settings for dealing with rea...2313 days ago
flexidot: Highly customizable, ambiguity-aware dotplots for visual sequence analyses
FlexiDot is a cross-platform dotplot suite generating high quality self, pairwise and all-against-all visualizations. To improve dotplot suitability for comparison of co...1514 days ago
BBSplit: Read Binning Tool for Metagenomes and Contaminated Libraries
...es not matter whether they map ambiguously within human, only whether they are ambiguous between human and mouse. BBSplit tracks this additional ambiguity information and decides how t...2357 days ago
3347 days ago
Create genome scaffolding with Perl
...", AG => "R", AR => "A", GR => "G", CT => "Y", CY => "C", TY => "T", AT => "W", AW => "A", TW => "T", ); # if "simple" ambiguity can be found, return that, ot...2351 days ago
Comment on "GraphMap - A highly sensitive and accurate mapper for long, error-prone reads"
...lap - Equivalent to: '-a anchor -w normal --overlapper --evalue 1e0 --ambiguity 0.50 --secondary' sensitive -..., --circular - Reference sequence is a circular genome. [false] -F, --ambiguity FLT All mapping positions wit...2334 days ago