RNA-Seq De novo Assembly Using Trinity
Trinity, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. Trinity combines three independent software modules: Inchworm, Chrysalis, and Butterfly, applied seq...Tags: Bioinformatics, NGS, RNA-seq, Assembly, Trinity, Illumina
2966 days ago
Tags: Bioinformatics, NGS, Assembly, View, Fig, Easyfig, Tool
2929 days ago
PAired-eND Assembler for DNA sequences
PANDASEQ is a program to align Illumina reads, optionally with PCR primers embedded in the sequence, and reconstruct an overlapping sequence. More at https://github.com/neufeld/pandaseqTags: Bioinformatics, NGS, Assembly, Paired-End, PANDASEQ, Illumina
2952 days ago
Tags: Bioinformatics, NGS, Assembly, Paired-End, MIRA, Illumina
2952 days ago
RACA: Reference-Assisted Chromosome Assembly
Rreference-Assisted Chromosome Assembly (RACA), an algorithm to reliably order and orient sequence scaffolds generated by NGS and assemblers into longer chromosomal fragments using comparative genome information and paired-end reads. http://www.ncbi.nlm.nih.gov/pubmed/23307812 http://bioen-comp...Tags: Bioinformatics, NGS, Assembly, Reference, RACA, Reads
2952 days ago
QUAST: quality assessment tool for genome assemblies
QUAST evaluates genome assemblies. For metagenomes, please see MetaQUAST project.It can works both with and without a given reference genome.The tool accepts multiple assemblies, thus is suitable for comparison. More at http://bioinf.spbau.ru/quast http://bioinformatics.oxfordjou...Tags: Bioinformatics, Computational Biology, Assembly, Comparison, Accuracy
2951 days ago
REAPR: a universal tool for genome assembly evaluation
REAPR is a tool that evaluates the accuracy of a genome assembly using mapped paired end reads, without the use of a reference genome for comparison. It can be used in any stage of an assembly pipeline to automatically break incorrect scaffolds and flag other errors in an assembly for manual insp...Tags: Bioinformatics, Computational Biology, Assembly, Comparison, Accuracy, Evaluation
2951 days ago
Tags: Bioinformatics, NGS, Assembly, Quality, FastQC, Tutorial
2943 days ago
Tags: Bioinformatics, NGS, Assembly, DISCOVAR, Variants, BroadInstitute
2939 days ago