Results for "Assembly"

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  • Picard

    Picard is a set of command line tools for manipulating high-throughput sequencing (HTS) data and formats such as SAM/BAM/CRAM and VCF. These file formats are defined in the Hts-specs repository. See especially the SAM specification and the VCF specification. Note that the information on this pag...

    Tags: Bioinformatics, NGS, Assembly, BAM, SAM, CRAM, Picard, FastQC, Tool, Software

    2933 days ago

  • RNA-Seq De novo Assembly Using Trinity

    Trinity, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. Trinity combines three independent software modules: Inchworm, Chrysalis, and Butterfly, applied seq...

    Tags: Bioinformatics, NGS, RNA-seq, Assembly, Trinity, Illumina

    2970 days ago

  • Easyfig

    Easyfig has moved to github, for newer releases of Easyfig please visit our new webpage - https://mjsull.github.io/Easyfig.  Easyfig is a Python application for creating linear comparison figures of multiple genomic loci with an easy-to-use graphical user interface (GUI). More at http://eas...

    Tags: Bioinformatics, NGS, Assembly, View, Fig, Easyfig, Tool

    2933 days ago

  • #ConPADE: Genome #Assembly Ploidy Estimation from #Next-Generation Sequencing Data http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1004229

    Tags: ConPADE, Assembly, Next

    2957 days ago

  • PAired-eND Assembler for DNA sequences

    PANDASEQ is a program to align Illumina reads, optionally with PCR primers embedded in the sequence, and reconstruct an overlapping sequence.   More at https://github.com/neufeld/pandaseq

    Tags: Bioinformatics, NGS, Assembly, Paired-End, PANDASEQ, Illumina

    2956 days ago

  • Sequence assembly with MIRA 4

    MIRA is a multi-pass DNA sequence data assembler/mapper for whole genome and EST/RNASeq projects. MIRA assembles/maps reads gained by electrophoresis sequencing (aka Sanger sequencing) 454 pyro-sequencing (GS20, FLX or Titanium) Ion Torrent Solexa (Illumina) sequencing ...

    Tags: Bioinformatics, NGS, Assembly, Paired-End, MIRA, Illumina

    2956 days ago

  • RACA: Reference-Assisted Chromosome Assembly

    Rreference-Assisted Chromosome Assembly (RACA), an algorithm to reliably order and orient sequence scaffolds generated by NGS and assemblers into longer chromosomal fragments using comparative genome information and paired-end reads. http://www.ncbi.nlm.nih.gov/pubmed/23307812 http://bioen-comp...

    Tags: Bioinformatics, NGS, Assembly, Reference, RACA, Reads

    2956 days ago

  • QUAST: quality assessment tool for genome assemblies

    QUAST evaluates genome assemblies. For metagenomes, please see MetaQUAST project.It can works both with and without a given reference genome.The tool accepts multiple assemblies, thus is suitable for comparison. More at http://bioinf.spbau.ru/quast http://bioinformatics.oxfordjou...

    Tags: Bioinformatics, Computational Biology, Assembly, Comparison, Accuracy

    2956 days ago

  • REAPR: a universal tool for genome assembly evaluation

    REAPR is a tool that evaluates the accuracy of a genome assembly using mapped paired end reads, without the use of a reference genome for comparison. It can be used in any stage of an assembly pipeline to automatically break incorrect scaffolds and flag other errors in an assembly for manual insp...

    Tags: Bioinformatics, Computational Biology, Assembly, Comparison, Accuracy, Evaluation

    2956 days ago

  • Understanding Fastqc Output

    Understanding Following table and graphs Duplication level kmer profile per base GC content per base N content per base quality per base sequence content per sequence GC content per sequence quality sequence length distribution More at http://www.bioinformatics.babraham.ac.uk/projec...

    Tags: Bioinformatics, NGS, Assembly, Quality, FastQC, Tutorial

    2947 days ago