Ancestral sequence reconstruction steps !
Ancestral sequence reconstruction (ASR) – also known as ancestral gene/sequence reconstruction/resurrection – is a techniq...BEAST is the best way to predict the ancestral structure. but...ion on Windows in "http://web.cbio.uct.ac.za/~darren/rdp.html...2192 days ago
Gap filling or Contigs extensions tools !
...ler: a de novo assembly approach to fill the gap within paired reads" or "Toward almost closed genomes with GapFiller"....it is useful only in some specific situations. Take a loo...art with the initial assembly C0 of the contigs you have iden...2178 days ago
LINKS scaffolder bloomfilter setting !
...oomfilter git:(master) ✗ g++ -c BloomFilter_wrap.cxx -I/home/...➜ bloomfilter git:(master) ✗ cd swig ➜ swig git:(master)...o -O3 ➜ swig git:(master) ✗ cd .. ➜ bloomfilter git:(mast...error (%) allowed on -d distance e.g. -e 0.1 == distance +...2164 days ago
Understanding BLASTn output format 6 !
...t DNA, for example gene sequences against a reference genomeblastn -query genes.f...e.g., reference genome) sequence id 3. pident perce... expect value 12. bitscore bit score Defin...enceevalue Expect valuebitscore Bit scorescore Raw sc...2152 days ago
Interview Puzzles for Bioinformatician !
...erview Puzzles being asked in top tech companies.Here is a list of Top...Interview. 2 Eggs and 100 Floor Classic Puzzle Five pirates an...om Airplane Seats Inverted playing card puzzle Flipping Coins Puz...Burning ropes to measure time Connect 3 houses with 3 wells Measu...2132 days ago
My commonly used commands in Bioinformatics
...YI, I've found it useful to use MUMmer to extract the specific changes that Racon makes,...p -t 24 assembly.fasta long_reads.fastq.gz | racon -t 24 long_reads.fastq.gz -...ssembly.fasta racon_assembly.fasta show-snps -C -T -r nucmer.delta This re...2123 days ago
List of non-commercial NGS genotype-calling software
...ysis of next-generation sequencing (NGS) data, which are produced extensively by genetics and genomics studies, relies crucially on...ulation of NGS alignments, which includes a computation of ge...Software for SNP and genotype calling, including a method for...2109 days ago
Parallel Processing with Perl !
...make best use of multiple processors for bioinformatics analysis. One best way is us...ing; } This is very slow(since we are using only one proces...rt and next; # do the forkyou code here;$fork->finish; # d...l_children; So basically each processor will be active wit...2093 days ago
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