Understanding reads mapping and flags !
...f alignment on reverse strand). Chimeric Alignment: An alignment...ne of the linear alignments in a chimeric alignment is considered the &...enes in RNA-seq. In short, chimeric reads can be split in to two...Supplementary Alignment: A chimeric reads but not a representativ...1852 days ago
Bioinformatics tools for telomere to telomere assembly !
...nd other low-complexity gene amplicon data; performs allele calling while detecting polymorphic sites within the sequences and removing potential chimeric sequence variants (Yuanyuan C...1006 days ago
2946 days ago
Bioinformatics tools and software
.... UPARSE >OTU clustering for 16S and other marker genes. Highly accurate OTU sequences and improved diversity measures. UCHIME >Chimeric sequence detection. PILE...2876 days ago
Genome Assembly Tools and Software - PART1 !!
...nome based on a reference genome. VTBuilder – Assembly of Multi Isoform Transcriptomes VTBuilder is a tool for the inference of non-chimeric contigs from read data that h...2700 days ago
2090 days ago
Understanding reads mapping and flags !
Linear Alignment: An alignment of a read to a single reference sequence that may include insertions, deletions, skips and clipping, but may not include direction changes (i.e. one portion of the alignment on forward strand and another portion of alignment on reverse strand). ...Tags: Linear, Chimeric, Supplementary, Primary, Secondary, Alignments
1852 days ago