Useful Publications and Websites for Deep Sequencing Data Analysis
Global overview papers Next generation quantitative genetics in plants. Jiménez-Gómez, Frontiers in Plant Science 2:77, 2011 Full Text [equally relevant to animal and microbial systems] Sense from sequence reads: methods for alignment and assembly. Flicek & Birney, Nat Methods...Tags: Bioinformatics, Genomics, NGS, Deep Sequencing
3772 days ago
Perl one-liner for bioinformatician !!!
With the emergence of NGS technologies, and sequencing data most of the bioinformaticians mung and wrangle around massive amounts of genomics text. There are several "standardized" file formats (FASTQ, SAM, VCF, etc.) and some tools for manipulating them (fastx toolkit, samtools, vcftools, etc.),...Tags: Bioinformatics, Computational Biology, Perl, NGS, Genome, Hacking, One-liner, Oneliner
3621 days ago
Next generation sequencing in R or bioconductor environment
There are many R software and bioconductor packages for NGS data analysis, some of them are as follows Biostrings The Biostrings package from Bioconductor provides an advanced environment for efficient sequence management and analysis in R. It contains many speed and memory effective string con...Tags: Bioinformatics, Computational Biology, NGS, R, Bioconductor, Packages, Modules, Next generation sequencing
3618 days ago
Commercial and public next-gen-seq (NGS) software
Integrated solutions CLCbio Genomics Workbench - de novo and reference assembly of Sanger, Roche FLX, Illumina, Helicos, and SOLiD data. Commercial next-gen-seq software that extends the CLCbio Main Workbench software. Includes SNP detection, CHiP-seq, browser and other features. Commercial. Wind...Tags: Bioinformatics, Computational Biology, NGS, Next generation sequencing, Tools, Software, SNP, Assembly, Pipeline
3512 days ago
List of Bioinformatics Software Tools for Next Generation Sequencing
Commercial tools Strand NGS offers many different tools including alignment, RNA-Seq, DNA-Seq, ChIP-Seq, Small RNA-Seq, Genome Browser, visualizations, Biological Interpretation, etc. Supports workflows “one can import the sample data in FASTA, FASTQ or tag-count format. In addition, ...Tags: Bioinformatics, Computational Biology, Tools, Software, List, NGS
2902 days ago
Tags: Bioinformatics, BBMap/BBTools package, Multipurpose, Tool, Converting, Formats, NGS
2707 days ago
Tags: Bioinformatics, Glossary, NGS
2862 days ago
Genome Assembly Tools and Software - PART1 !!
The genome assemblers generally take a file of short sequence reads and a file of quality-value as the input. Since the quality-value file for the high throughput short reads is usually highly memory-intensive, only a few assemblers, best suited for your assembly. For the sake of computational me...Tags: Bioinformatics, Genome, NGS, Assembly, Tools, Visualization
2679 days ago
Quick next generation sequencing (NGS) terms definition
fragment size: the Illumina WGS protocol generates paired-end reads from both ends of longer fragments. The lengths of these fragments are assumed to be sampled from a normal distribution. Therefore, in the absence of structural variants, mapping locations of the paired ends span within an i...Tags: Quick, NGS, definition, fragment size, concordant reads, discordant reads, split reads, read depth
2515 days ago
List of visualization tools for genome alignments
Genome browsers are useful not only for showing final results but also for improving analysis protocols, testing data quality, and generating result drafts. Its integration in analysis pipelines allows the optimization of parameters, which leads to better results. But sometime, we need publi...Tags: List, visualization, tools, genome, alignments, NGS
2277 days ago