Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant calling Read alignme...874 days ago
A Post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs
PAGIT addresses the need for software to generate high quality draft genomes. It is b...ion from a reference genome, or an earlier assembly, onto the latest assembly. PAGIT bundles these software and ma...2547 days ago
Genome Assembly Tools and Software - PART2 !!
...ldquo;only” at closing the gap between pairs of reads as a first step of a large number of downstream analysis PAGIT 1.01 – Post Assembly Genome Improvement ToolkitPAGIT (Post Assembly Genome Im...2683 days ago
Tools for bacterial whole genome annotation
...e mode. BlastKOALA is suitable for annotating fully sequenced genomes. PAGIT: Provides a toolkit for impro...ving the quality of genome assemblies created via an assembly software. PAGIT compiled four tools: (i) ABAC...2329 days ago
Gap filling or Contigs extensions tools !
...bcm.tmc.edu/content/bcm-hgsc-software), and SCARPA (http://compbio.cs.toronto.edu/hapsembler/scarpa.html). See the PAGIT protocol: http://www.sanger.ac.uk/resources/software/pagit/ In particular, take a lo...2162 days ago
A Post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs
PAGIT addresses the need for software to generate high quality draft genomes. It is based on a series of programs that we developed: ABACAS, that is able to contiguate contigs from a de novo assembly against a closely related reference. IMAGE, an iterative approach for closing gaps in assembled...Tags: Bioinformatics, Alignment, Assembly, PAGIT, ABACAS, iCRON, RATT, IMAGE
2547 days ago