Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant calling Read alignme...878 days ago
A Post-assembly genome-improvement toolkit (PAGIT) to obtain annotated genomes from contigs
PAGIT addresses the need for software to generate high quality draft genomes. It is b...ion from a reference genome, or an earlier assembly, onto the latest assembly. PAGIT bundles these software and ma...2551 days ago
Genome Assembly Tools and Software - PART2 !!
...ldquo;only” at closing the gap between pairs of reads as a first step of a large number of downstream analysis PAGIT 1.01 – Post Assembly Genome Improvement ToolkitPAGIT (Post Assembly Genome Im...2686 days ago
Tools for bacterial whole genome annotation
...e mode. BlastKOALA is suitable for annotating fully sequenced genomes. PAGIT: Provides a toolkit for impro...ving the quality of genome assemblies created via an assembly software. PAGIT compiled four tools: (i) ABAC...2332 days ago
Gap filling or Contigs extensions tools !
...bcm.tmc.edu/content/bcm-hgsc-software), and SCARPA (http://compbio.cs.toronto.edu/hapsembler/scarpa.html). See the PAGIT protocol: http://www.sanger.ac.uk/resources/software/pagit/ In particular, take a lo...2166 days ago