Perl Special Vars Quick Reference
$_ The default or implicit variable. @_ Subroutine parameters. $a$b sort comparison routine variables. @ARGV The command-line args. Regular Expressions $<digit> Regexp parenthetical capture holders. $& Last successful match (degrades perfor...Tags: Perl; Special, Vars, Quick, Reference
2658 days ago
Quick next generation sequencing (NGS) terms definition
fragment size: the Illumina WGS protocol generates paired-end reads from both ends of longer fragments. The lengths of these fragments are assumed to be sampled from a normal distribution. Therefore, in the absence of structural variants, mapping locations of the paired ends span within an i...Tags: Quick, NGS, definition, fragment size, concordant reads, discordant reads, split reads, read depth
2536 days ago
A quick guide to Phred scaling
A quick guide to Phred scaling• Phred value = −10 * log10(ε)• Examples:• 90% confidence (10% error rate) = Q10• 99% confidence (1% error rate) = Q20• 99.9% confidence (.1% error rate) = Q30• SAM encoding adds 33 to the value (becauseASCII 33 is the firs...Tags: quick, guide, Phred, scaling
1548 days ago