Results for "R"

Top-level pages

  • Ancestral sequence reconstruction steps !

    Ancestral sequence reconstruction (ASR) – also known as ancestral gene/sequence reconstructio...sp;protein. The idea of protein 'resurrection' was sugge...ent of algorithms and of better sequencing and synthesis tech...c signal, is possible to perform with Tree-Puzzle "http://www...

    2173 days ago

  • Gap filling or Contigs extensions tools !

    There are many tools to perform gap filling using Illumina short reads, for example "GapFiller: a de novo assembly approach to fill the gap within pa...//genomebiology.com/2010/11/4/R41 Also SOAPdenovo has ha...ssembly C' from C and the new reads identified in (a) and (b)...

    2159 days ago

  • LINKS scaffolder bloomfilter setting !

    ➜ bin git:(master) ✗ ls -l total 68 drwxrwxr-x 3 urbe urbe 4096 Jun 15 12:15 lib -rwxrwxrwx 1 urbe urbe 65141 Jun...ter.so -O3 ➜ swig git:(master) ✗ cd .. ➜ bloomfilter git:...minimum number of links (k-mer pairs) to compute scaffold (d...

    2145 days ago

  • Understanding BLASTn output format 6 !

    BLASTn output format 6 BLASTn maps DNA against DNA, for example gene sequences against a reference genomeblastn  -query ...xpect value  12.  bitscore  bit score Define...ceevalue    Expect valuebitscore  Bit scorescore     Raw scor...

    2132 days ago

  • Interview Puzzles for Bioinformatician !

    These are some of the most famous Interview Puzzles being asked in top tech companies.Here is a list of Top 25 puzzles...ve been asked in top Tech Interview. 2 Eggs and 100 Floor...itches Find the minimum number of aircraft Burning ropes to...

    2113 days ago

  • My commonly used commands in Bioinformatics

    ...I, I've found it useful to use MUMmer to extract the specific changes that...minimap -t 24 assembly.fasta long_reads.fastq.gz | racon -t 24 lo...ly.fasta racon_assembly.fasta nucmer -p nucmer assembly.fasta raco...MUMmer -> SNP table) solves the problem I originally raised in t...

    2104 days ago

  • List of non-commercial NGS genotype-calling software

    ...aningful analysis of next-generation sequencing (NGS) data, which are produced extensively by genetics and genomics studies, relies crucially on the accurat...(bcftools) GATK http://www.broadinstitute.org/gsa/wiki/inde...rmation (NLDA) and a method for incorporating LD information...

    2090 days ago

  • Parallel Processing with Perl !

    Here is a small tutorial on how to make best use of multiple processors for bioinformatics an...;  push (@childs,$fork); }elseif($fork==0){you..._processors=8;my $fork= new Parallel::ForkManager($max_proces...iece of $file;######### end fork ###############$super_fork-&...

    2073 days ago

  • Benchmarking Perl Module !

    The benchmark module is a great tool to know the time the code takes to run. The output is usually in terms of CPU time. This module provides us with a way to optimi...about the CPU time taken by our perl program. This is the si...nt “the code took, timestr($final_difference),”\n&...

    2073 days ago

  • Installing BLAT on Linux !

    ...when I went to the download directory at UCSC: http://users.s...u/admin/exe/ and that there was a linux x86_64 executabl...t part of the MACHTYPE). To correct this, in the bash shell t...npacked blat into).Now simply run the command: make to comp...

    2057 days ago