1830 days ago
Bioinformatics tools for telomere to telomere assembly !
● Merfin – k-mer-based assembly and variant c...enerate high-quality consensus sequences from HiFi reads (Zev Kronenberg @zevkron...; tool for splice alignment of long transcriptomic reads to a genome, guided by a data...986 days ago
Protocol for De novo Genome Assembly using Illumina Reads
In this protocol, we address and describe the de...on the sequencing system used, these "reads" range from 20 to 1000 nucleo...pairs (bp) in length. Usually, length reads of 36 - 150 bp are produced f...for infectious diseases. Raw NGS dataReads can be saved as a Fasta file...1198 days ago
Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant...871 days ago
Useful Bioinformatics Analysis Tools !
CoMeta Classificier of reads from metagenomic sequencing experiments. &bull...):22–234, DSRC Compressor of sequencing reads. • Roguski,..., ORCOM Experimental compressor of sequencing reads. • Grabowski...857 days ago
Common steps for reads mapping !
Mapping reads to a reference genome is an essent...eneral steps to follow for mapping reads to a genome: Choose a re...e reference genome: Before mapping reads, the reference genome needs t...mapping rate, the number of mapped reads, and the mapping quality scor...417 days ago
Steps to find palindrome in genomes !
Palindromes are sequences of nucleotides...h as PacBio and Oxford Nanopore, can generate long reads that can span entire palindromic regions. By mapping these reads to the genome, palindromic re...417 days ago