Software and Tools to detect structure variation with long reads !!
...p;Single Molecule, Real-Time (SMRT) Sequencing, you can access str...third generation sequencing (PacBio or Oxford Nanopore). It detec...forge.net/projects/pb-jelly/ SMRT-SV: Structural variant and indel caller for PacBio reads Structural variant (...2609 days ago
BBTools for bioinformatician !
BBMap.sh Mapping Nanopore reads BBMap.sh has a length cap of 6kbp. R...mapped independently. Code: $ mapPacBio.sh -Xmx20g k=7 in=reads.fastq...ed on the basis of quality; there's a PacBio error model; and all of the r...ed on the basis of quality; there's a PacB...2272 days ago
Understating pacbio reads name !
m140415_143853_42175_c100635972550000001823121909121417_s1_p0/553/3100_11230 0.99 24..."m" = movie Time of Run Start (yymmdd_hhmmss) Instrument Serial Number SMRT Cell Barcode Set Number (a.k...1991 days ago
CANU genome assembly parameters !
Choose the appropriate parameters to run Canu and run it. The assembly will take about an hour. You can use two...sm_test3 \ genomeSize=2m \ -maxThreads=2 useGrid=false \ -pacbio-raw \ ~/pacb...1946 days ago
Bioinformatics tools for telomere to telomere assembly !
...-read transcriptome data (Richard Kuo @GenomeRIK)● pbaa (PacBio Amplicon Analysis) – se...igators-share-stellar-science-career-advice-and-bioinformatics-tools-at-smrt-leiden-2021/993 days ago
Steps to find palindrome in genomes !
Palindromes are sequences of nucleotides that read the same backward as forward. They can be pre...Next-generation sequencing: High-throughput sequencing technologies, such as PacB...425 days ago