Software and Tools to detect structure variation with long reads !!
...p;Single Molecule, Real-Time (SMRT) Sequencing, you can access str...third generation sequencing (PacBio or Oxford Nanopore). It detec...forge.net/projects/pb-jelly/ SMRT-SV: Structural variant and indel caller for PacBio reads Structural variant (...2609 days ago
BBTools for bioinformatician !
BBMap.sh Mapping Nanopore reads BBMap.sh has a length cap of 6kbp. R...mapped independently. Code: $ mapPacBio.sh -Xmx20g k=7 in=reads.fastq...ed on the basis of quality; there's a PacBio error model; and all of the r...ed on the basis of quality; there's a PacB...2272 days ago
Bioinformatics algorithms tutorials
Useful bioinformatics tutorial, such as De Bruijn Graphs for NGS AssemblyAlgorithms for PacBio ReadsSoftware and Hardware Concepts for BioinformaticsFinding us in Homolog.us (Search Algorithms)NG...3605 days ago
GraphMap - A highly sensitive and accurate mapper for long, error-prone reads
GraphMap is a novel mapper targeted at aligning long, error-prone third-generation seque...gnificant improvement over the state-of-the-art for PacBio read mappers. GraphMap was a...and error profiles, including: Illumina, PacB...3226 days ago
825 days ago
Genome Assembly Tools and Software - PART2 !!
The genome assemblers generally take a file of short sequence reads and a file of qualit...Sanger, 454, Solexa (Illumina), IonTorrent data and PacBio (the later at the moment only...-error-correction (MEC) model. PBSIM 1.0.3 – PacB...2687 days ago
Genome Assembly Tools and Software - PART1 !!
...from Long Gapless Reads A fast and memory-efficient method for haplotype assembly from long gapless reads, like those produced by SMRT sequencing technologies (PacBio RS II) and Oxford Nanopore...2687 days ago
Alignment-free sequence comparison tools available for next-generation sequencing data analysis
.../MHAP Miniasm Assembler of long noisy reads (SMRT, ONT) using the Overlap-Layout...h error-containing long sequence (e.g., ONT or PacBio reads, draft genomes) Softwa...ation using assembled or unassembled Illumina, PacBio, and ONT data (based on Min...2372 days ago
Phased Human Genome Assembly !
...publicly available assembly (PacBio HG00733) has the fewest...tigs that were assembled with PacBio data generated with the compa...assembly are included on the PacBio blog. The data are available...details are available on the PacBio website: Blog post: D...2037 days ago
Genome + Epigenome = New Understanding of the Pathogens in Your Food
UC Davis's Bart Weimer describes foodborne pathogens and their proclivity for rapid genome rearrangement. The 100K Pathogen Genome Project he leads is using PacB...3874 days ago
2253 days ago
Long reads mapper bash script !
#!/bin/bash #only for LONG READS mapping #USAGE: runMapper.sh minimap2 ref.fa reads.fa/fq 48 ont...d $refFasta $longReads > $fileName.out.sam elif [ $readsType == "pacbio" ]; then $lamsaLoc aln -t $thread -T pacb...2124 days ago
Single molecule Sequencing (SMS)
...ntists now keen to use SMS method to sequence molecules in comparatively less complex protocal and less time. One of most successful so far is SMRT from Pacbio and others will be flood th...3228 days ago
Single molecule Sequencing (SMS)
SMS is now slowly gaining place in sequencing world, more and more scientists now keen to use SMS method to sequence molecules in comparatively less complex protocal and less time. One of most successful so far is SMRT from Pacbio and others will be flood the market soon and may be one day it rep...Tags: Single molecule Sequencing, nanopore, pacbio smrt, DNA
3228 days ago
Comment on "LAMSA: fast split read alignment with long approximate matches"
...hin reads. [0.04] -T --read-type [STR] Specifiy the type of reads and set multiple parameters unless overriden. [null] (Illumina Moleculo) pacbio (PacBio SMRT): -i25 -l50 -m1 -M1 -O1,1,2,2...2124 days ago