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Running Trinity on RNA-seq !

  • Public
By Jit 2330 days ago Comments (1)
➜ trinityrnaseq-Trinity-v2.6.6 git:(master) ✗ ./Trinity --seqType fq --max_memory 250G --left /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq --right /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq --CPU 40 ______ ____ ____ ____ ____ ______ __ __ | || \ | || \ | || || | | | || D ) | | | _ | | | | || | | |_| |_|| / | | | | | | | |_| |_|| ~ | | | | \ | | | | | | | | | |___, | | | | . \ | | | | | | | | | | | |__| |__|\_||____||__|__||____| |__| |____/ Left read files: $VAR1 = [ '/media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq' ]; Right read files: $VAR1 = [ '/media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq' ]; Trinity version: Trinity-v2.6.6 ** NOTE: Latest version of Trinity is Trinity-v2.7.0-PRERELEASE, and can be obtained at: https://github.com/trinityrnaseq/trinityrnaseq/releases Monday, July 9, 2018: 10:35:42 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/ExitTester.jar 0 Monday, July 9, 2018: 10:35:42 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/ExitTester.jar 1 Monday, July 9, 2018: 10:35:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir Monday, July 9, 2018: 10:35:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis ---------------------------------------------------------------------------------- -------------- Trinity Phase 1: Clustering of RNA-Seq Reads --------------------- ---------------------------------------------------------------------------------- --------------------------------------------------------------- ------------ In silico Read Normalization --------------------- -- (Removing Excess Reads Beyond 50 Coverage -- --------------------------------------------------------------- # running normalization on reads: $VAR1 = [ [ '/media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq' ], [ '/media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq' ] ]; Monday, July 9, 2018: 10:35:42 CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/insilico_read_normalization.pl --seqType fq --JM 250G --max_cov 50 --min_cov 1 --CPU 40 --output /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization --max_pct_stdev 10000 --left /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq --right /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq --pairs_together --PARALLEL_STATS Converting input files. (both directions in parallel)CMD: seqtk-trinity seq -A /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq >> left.fa CMD: seqtk-trinity seq -A /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq >> right.fa CMD finished (128 seconds) CMD finished (129 seconds) CMD: touch left.fa.ok CMD finished (0 seconds) CMD: touch right.fa.ok CMD finished (0 seconds) Done converting input files.CMD: cat left.fa right.fa > both.fa CMD finished (5 seconds) CMD: touch both.fa.ok CMD finished (0 seconds) ------------------------------------------- ----------- Jellyfish -------------------- -- (building a k-mer catalog from reads) -- ------------------------------------------- CMD: jellyfish count -t 40 -m 25 -s 100000000 --canonical both.fa CMD finished (33 seconds) CMD: jellyfish histo -t 40 -o jellyfish.K25.min2.kmers.fa.histo mer_counts.jf CMD finished (6 seconds) CMD: jellyfish dump -L 2 mer_counts.jf > jellyfish.K25.min2.kmers.fa CMD finished (16 seconds) CMD: touch jellyfish.K25.min2.kmers.fa.success CMD finished (0 seconds) CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads left.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 20 --DS > left.fa.K25.stats CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads right.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 20 --DS > right.fa.K25.stats -reading Kmer occurrences...-reading Kmer occurrences... done parsing 59324464 Kmers, 59324464 added, taking 99 seconds. done parsing 59324464 Kmers, 59324464 added, taking 113 seconds. STATS_GENERATION_TIME: 252 seconds. CMD finished (378 seconds) STATS_GENERATION_TIME: 299 seconds. CMD finished (411 seconds) CMD: touch left.fa.K25.stats.ok CMD finished (0 seconds) CMD: touch right.fa.K25.stats.ok CMD finished (0 seconds) -sorting each stats file by read name. CMD: /usr/bin/sort --parallel=40 -k5,5 -T . -S 125G left.fa.K25.stats > left.fa.K25.stats.sort CMD: /usr/bin/sort --parallel=40 -k5,5 -T . -S 125G right.fa.K25.stats > right.fa.K25.stats.sort CMD finished (9 seconds) CMD finished (9 seconds) CMD: touch left.fa.K25.stats.sort.ok CMD finished (0 seconds) CMD: touch right.fa.K25.stats.sort.ok CMD finished (0 seconds) CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/..//util/support_scripts//nbkc_merge_left_right_stats.pl --left left.fa.K25.stats.sort --right right.fa.K25.stats.sort --sorted > pairs.K25.stats -opening left.fa.K25.stats.sort -opening right.fa.K25.stats.sort -done opening files. CMD finished (85 seconds) CMD: touch pairs.K25.stats.ok CMD finished (0 seconds) CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/..//util/support_scripts//nbkc_normalize.pl --stats_file pairs.K25.stats --max_cov 50 --min_cov 1 --max_pct_stdev 10000 > pairs.K25.stats.C50.pctSD10000.accs 6275795 / 16641634 = 37.71% reads selected during normalization. 0 / 16641634 = 0.00% reads discarded as likely aberrant based on coverage profiles. 0 / 16641634 = 0.00% reads missing kmer coverage (N chars included?). 0 / 16641634 = 0.00% reads discarded as below minimum coverage threshold=1 CMD finished (25 seconds) CMD: touch pairs.K25.stats.C50.pctSD10000.accs.ok CMD finished (0 seconds) CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq.ok CMD finished (0 seconds) CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq.ok CMD finished (0 seconds) CMD: ln -sf /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq left.norm.fq CMD finished (0 seconds) CMD: ln -sf /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq right.norm.fq CMD finished (0 seconds) -removing tmp dir /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/tmp_normalized_reads Normalization complete. See outputs: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq Monday, July 9, 2018: 10:51:26 CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/normalization.ok Converting input files. (in parallel)Monday, July 9, 2018: 10:51:26 CMD: cat /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/left.norm.fq | seqtk-trinity seq -A - >> left.fa Monday, July 9, 2018: 10:51:26 CMD: cat /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/right.norm.fq | seqtk-trinity seq -A - >> right.fa Monday, July 9, 2018: 10:51:30 CMD: touch left.fa.ok Monday, July 9, 2018: 10:51:30 CMD: touch right.fa.ok Monday, July 9, 2018: 10:51:30 CMD: touch left.fa.ok right.fa.ok Monday, July 9, 2018: 10:51:30 CMD: cat left.fa right.fa > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa Monday, July 9, 2018: 10:51:32 CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa.ok ------------------------------------------- ----------- Jellyfish -------------------- -- (building a k-mer catalog from reads) -- ------------------------------------------- * Running CMD: jellyfish count -t 40 -m 25 -s 100000000 --canonical /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa * Running CMD: jellyfish dump -L 1 mer_counts.jf > jellyfish.kmers.fa * Running CMD: jellyfish histo -t 40 -o jellyfish.kmers.fa.histo mer_counts.jf ---------------------------------------------- --------------- Inchworm --------------------- -- (Linear contig construction from k-mers) -- ---------------------------------------------- * Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/bin//inchworm --kmers jellyfish.kmers.fa --run_inchworm -K 25 -L 25 --monitor 1 --DS --num_threads 6 --PARALLEL_IWORM > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa.tmp * Running CMD: mv /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa.tmp /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa Monday, July 9, 2018: 11:00:35 CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa.finished -------------------------------------------------------- -------------------- Chrysalis ------------------------- -- (Contig Clustering & de Bruijn Graph Construction) -- -------------------------------------------------------- inchworm_target: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa bowite_reads_fa: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa chrysalis_reads_fa: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa * Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/filter_iworm_by_min_length_or_cov.pl /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa 100 10 > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/inchworm.K25.L25.DS.fa.min100 * Running CMD: /home/urbe/anaconda3/bin/bowtie2-build --threads 40 -o 3 /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/inchworm.K25.L25.DS.fa.min100 /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/inchworm.K25.L25.DS.fa.min100 1>/dev/null * Running CMD: bash -c " set -o pipefail;/home/urbe/anaconda3/bin/bowtie2 --local -k 2 --no-unal --threads 40 -f --score-min G,20,4 -x /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/inchworm.K25.L25.DS.fa.min100 /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa | samtools view -@ 40 -F4 -Sb - | samtools sort -m 3355443200 -@ 40 -no - - > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm.bowtie.nameSorted.bam" * Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/scaffold_iworm_contigs.pl /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm.bowtie.nameSorted.bam /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm_scaffolds.txt * Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis/GraphFromFasta -i /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa -r /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa -min_contig_length 200 -min_glue 2 -glue_factor 0.05 -min_iso_ratio 0.05 -t 40 -k 24 -kk 48 -scaffolding /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm_scaffolds.txt > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm_cluster_welds_graph.txt * Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis/BubbleUpClustering -i /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa -weld_graph /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm_cluster_welds_graph.txt -min_contig_length 200 > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/GraphFromIwormFasta.out * Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis/CreateIwormFastaBundle -i /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/GraphFromIwormFasta.out -o /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/bundled_iworm_contigs.fasta -min 200 * Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis/ReadsToTranscripts -i /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa -f /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/bundled_iworm_contigs.fasta -o /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/readsToComponents.out -t 40 -max_mem_reads 50000000 * Running CMD: /usr/bin/sort --parallel=40 -T . -S 250G -k 1,1n /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/readsToComponents.out > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/readsToComponents.out.sort Monday, July 9, 2018: 11:17:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_0 Monday, July 9, 2018: 11:17:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_1 Monday, July 9, 2018: 11:17:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_2 Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_3 Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_4 Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_5 Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_6 Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_7 Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_8 Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_9 Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_10 Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_11 Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_12 Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_13 Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_14 Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_15 Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_16 Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_17 Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_18 Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_19 Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_20 Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_21 Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_22 Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_23 Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_24 Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_25 Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_26 Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_27 Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_28 Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_29 Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_30 Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_31 Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_32 Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_33 Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_34 Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_35 Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_36 Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_37 Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_38 Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_39 Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_40 Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_41 Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_42 Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_43 Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_44 Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_45 Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_46 Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_47 Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_48 Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_49 Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_50 Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_51 Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_52 Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_53 Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_54 Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_55 Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_56 Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_57 Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_58 Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_59 Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_60 Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_61 Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_62 Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_63 Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_64 Monday, July 9, 2018: 11:17:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_65 Monday, July 9, 2018: 11:17:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_66 Monday, July 9, 2018: 11:17:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_67 Monday, July 9, 2018: 11:17:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_68 Monday, July 9, 2018: 11:17:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_69 Monday, July 9, 2018: 11:17:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_70 Monday, July 9, 2018: 11:17:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_71 Monday, July 9, 2018: 11:17:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_72 Monday, July 9, 2018: 11:17:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_73 Monday, July 9, 2018: 11:17:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_74 Monday, July 9, 2018: 11:17:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_75 Monday, July 9, 2018: 11:17:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_76 Monday, July 9, 2018: 11:17:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_77 Monday, July 9, 2018: 11:17:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_78 Monday, July 9, 2018: 11:17:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_79 Monday, July 9, 2018: 11:17:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_80 Monday, July 9, 2018: 11:17:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_81 Monday, July 9, 2018: 11:17:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_82 Monday, July 9, 2018: 11:17:49 CMD: mkdir -p 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/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_139 Monday, July 9, 2018: 11:18:08 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_140 Monday, July 9, 2018: 11:18:08 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_141 Monday, July 9, 2018: 11:18:09 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_142 Monday, July 9, 2018: 11:18:09 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_143 Monday, July 9, 2018: 11:18:10 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_144 Monday, July 9, 2018: 11:18:10 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_145 Monday, July 9, 2018: 11:18:11 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_146 Monday, July 9, 2018: 11:18:12 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_147 Monday, July 9, 2018: 11:18:12 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_148 Monday, July 9, 2018: 11:18:13 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_149 Monday, July 9, 2018: 11:18:14 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_150 Monday, July 9, 2018: 11:18:14 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_151 Monday, July 9, 2018: 11:18:15 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_152 Monday, July 9, 2018: 11:18:16 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_153 Monday, July 9, 2018: 11:18:17 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_154 Monday, July 9, 2018: 11:18:17 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_155 Monday, July 9, 2018: 11:18:18 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_156 Monday, July 9, 2018: 11:18:19 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_157 Monday, July 9, 2018: 11:18:20 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_158 Monday, July 9, 2018: 11:18:20 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_159 Monday, July 9, 2018: 11:18:21 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_160 Monday, July 9, 2018: 11:18:22 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_161 Monday, July 9, 2018: 11:18:22 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_162 Monday, July 9, 2018: 11:18:22 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_163 Monday, July 9, 2018: 11:18:22 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_164 Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_165 Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_166 Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_167 Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_168 Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_169 Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_170 Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_171 Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_172 Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_173 Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_174 Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_175 Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_176 Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_177 Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_178 Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_179 Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_180 Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_181 Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_182 Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_183 Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_184 Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_185 Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_186 Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_187 Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_188 Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_189 Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_190 Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_191 Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_192 Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_193 Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_194 Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_195 Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_196 Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_197 Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_198 Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_199 Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_200 Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_201 Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_202 Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_203 Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_204 Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_205 Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_206 Monday, July 9, 2018: 11:18:31 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_207 Monday, July 9, 2018: 11:18:31 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_208 Monday, July 9, 2018: 11:18:31 CMD: mkdir -p 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/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_251 Monday, July 9, 2018: 11:18:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_252 Monday, July 9, 2018: 11:18:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_253 Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_254 Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_255 Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_256 Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_257 Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_258 Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_259 Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_260 Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_261 Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_262 Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_263 Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_264 Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_265 Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_266 Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_267 Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_268 Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_269 Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_270 Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_271 Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_272 Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_273 Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_274 Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_275 Monday, July 9, 2018: 11:18:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_276 Monday, July 9, 2018: 11:18:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_277 Monday, July 9, 2018: 11:18:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_278 Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_279 Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_280 Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_281 Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_282 Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_283 Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_284 Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_285 Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_286 Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_287 Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_288 Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_289 Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_290 Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_291 Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_292 Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_293 Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_294 Monday, July 9, 2018: 11:18:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_295 Monday, July 9, 2018: 11:18:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_296 Monday, July 9, 2018: 11:18:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_297 Monday, July 9, 2018: 11:18:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_298 Monday, July 9, 2018: 11:18:49 CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/partitioned_reads.files.list.ok Monday, July 9, 2018: 11:18:49 CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/write_partitioned_trinity_cmds.pl --reads_list_file /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/partitioned_reads.files.list --CPU 1 --max_memory 1G --run_as_paired --seqType fa --trinity_complete --full_cleanup > recursive_trinity.cmds Monday, July 9, 2018: 11:18:50 CMD: touch recursive_trinity.cmds.ok Monday, July 9, 2018: 11:18:50 CMD: touch recursive_trinity.cmds.ok -------------------------------------------------------------------------------- ------------ Trinity Phase 2: Assembling Clusters of Reads --------------------- -------------------------------------------------------------------------------- Monday, July 9, 2018: 11:18:50 CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/BIN/ParaFly -c recursive_trinity.cmds -CPU 40 -v -shuffle Number of Commands: 29867 succeeded(29867) 100% completed. All commands completed successfully. :-) ** Harvesting all assembled transcripts into a single multi-fasta file... Monday, July 9, 2018: 12:52:14 CMD: find /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/ -name '*inity.fasta' | /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/partitioned_trinity_aggregator.pl TRINITY_DN > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta.tmp -relocating /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta.tmp to /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta Monday, July 9, 2018: 12:52:15 CMD: mv /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta.tmp /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta ################################################################### Trinity assemblies are written to /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta ################################################################### Monday, July 9, 2018: 12:52:15 CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/get_Trinity_gene_to_trans_map.pl /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta.gene_trans_map

Comments

  • Jit 2326 days ago

    Once your assembly is complete, there are several analyses you will likely want to pursue to explore aspects of the biology of your organism based on your assembled transcripts and the input RNA-Seq data. Such studies might include:

    • Quantifying transcript and gene abundance. This is a prerequisite to many other analyses such as examining differentially expressed transcripts among your samples.

    • Quality checking your samples and biological replicates. Make sure the relationships among your samples and biological replicates make sense. If there are any confounding aspects to the data, such as outliers or batch effects, you'll want to catch them as early as possible and account for them in your further data explorations.

    • Perform differential expression analysis. Trinity provides direct support for several DE analysis methods, including edgeR, DESeq2, Limma/Voom, and ROTS.

    • Extract coding regions using TransDecoder and functionally annotate the transcripts using Trinotate.

    • If your organism has an assembled genome, consider using the Trinity transcriptome assemblies for gene structure annotation using PASA.