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Running Trinity on RNA-seq !
By Jit 2364 days ago Comments (1)
➜ trinityrnaseq-Trinity-v2.6.6 git:(master) ✗ ./Trinity --seqType fq --max_memory 250G --left /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq --right /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq --CPU 40
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Left read files: $VAR1 = [
'/media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq'
];
Right read files: $VAR1 = [
'/media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq'
];
Trinity version: Trinity-v2.6.6
** NOTE: Latest version of Trinity is Trinity-v2.7.0-PRERELEASE, and can be obtained at:
https://github.com/trinityrnaseq/trinityrnaseq/releases
Monday, July 9, 2018: 10:35:42 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/ExitTester.jar 0
Monday, July 9, 2018: 10:35:42 CMD: java -Xmx64m -XX:ParallelGCThreads=2 -jar /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/ExitTester.jar 1
Monday, July 9, 2018: 10:35:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir
Monday, July 9, 2018: 10:35:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis
----------------------------------------------------------------------------------
-------------- Trinity Phase 1: Clustering of RNA-Seq Reads ---------------------
----------------------------------------------------------------------------------
---------------------------------------------------------------
------------ In silico Read Normalization ---------------------
-- (Removing Excess Reads Beyond 50 Coverage --
---------------------------------------------------------------
# running normalization on reads: $VAR1 = [
[
'/media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq'
],
[
'/media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq'
]
];
Monday, July 9, 2018: 10:35:42 CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/insilico_read_normalization.pl --seqType fq --JM 250G --max_cov 50 --min_cov 1 --CPU 40 --output /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization --max_pct_stdev 10000 --left /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq --right /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq --pairs_together --PARALLEL_STATS
Converting input files. (both directions in parallel)CMD: seqtk-trinity seq -A /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq >> left.fa
CMD: seqtk-trinity seq -A /media/urbe/MyPassport/rnaseq_boris/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq >> right.fa
CMD finished (128 seconds)
CMD finished (129 seconds)
CMD: touch left.fa.ok
CMD finished (0 seconds)
CMD: touch right.fa.ok
CMD finished (0 seconds)
Done converting input files.CMD: cat left.fa right.fa > both.fa
CMD finished (5 seconds)
CMD: touch both.fa.ok
CMD finished (0 seconds)
-------------------------------------------
----------- Jellyfish --------------------
-- (building a k-mer catalog from reads) --
-------------------------------------------
CMD: jellyfish count -t 40 -m 25 -s 100000000 --canonical both.fa
CMD finished (33 seconds)
CMD: jellyfish histo -t 40 -o jellyfish.K25.min2.kmers.fa.histo mer_counts.jf
CMD finished (6 seconds)
CMD: jellyfish dump -L 2 mer_counts.jf > jellyfish.K25.min2.kmers.fa
CMD finished (16 seconds)
CMD: touch jellyfish.K25.min2.kmers.fa.success
CMD finished (0 seconds)
CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads left.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 20 --DS > left.fa.K25.stats
CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/..//Inchworm/bin/fastaToKmerCoverageStats --reads right.fa --kmers jellyfish.K25.min2.kmers.fa --kmer_size 25 --num_threads 20 --DS > right.fa.K25.stats
-reading Kmer occurrences...-reading Kmer occurrences...
done parsing 59324464 Kmers, 59324464 added, taking 99 seconds.
done parsing 59324464 Kmers, 59324464 added, taking 113 seconds.
STATS_GENERATION_TIME: 252 seconds.
CMD finished (378 seconds)
STATS_GENERATION_TIME: 299 seconds.
CMD finished (411 seconds)
CMD: touch left.fa.K25.stats.ok
CMD finished (0 seconds)
CMD: touch right.fa.K25.stats.ok
CMD finished (0 seconds)
-sorting each stats file by read name.
CMD: /usr/bin/sort --parallel=40 -k5,5 -T . -S 125G left.fa.K25.stats > left.fa.K25.stats.sort
CMD: /usr/bin/sort --parallel=40 -k5,5 -T . -S 125G right.fa.K25.stats > right.fa.K25.stats.sort
CMD finished (9 seconds)
CMD finished (9 seconds)
CMD: touch left.fa.K25.stats.sort.ok
CMD finished (0 seconds)
CMD: touch right.fa.K25.stats.sort.ok
CMD finished (0 seconds)
CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/..//util/support_scripts//nbkc_merge_left_right_stats.pl --left left.fa.K25.stats.sort --right right.fa.K25.stats.sort --sorted > pairs.K25.stats
-opening left.fa.K25.stats.sort
-opening right.fa.K25.stats.sort
-done opening files.
CMD finished (85 seconds)
CMD: touch pairs.K25.stats.ok
CMD finished (0 seconds)
CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/..//util/support_scripts//nbkc_normalize.pl --stats_file pairs.K25.stats --max_cov 50 --min_cov 1 --max_pct_stdev 10000 > pairs.K25.stats.C50.pctSD10000.accs
6275795 / 16641634 = 37.71% reads selected during normalization.
0 / 16641634 = 0.00% reads discarded as likely aberrant based on coverage profiles.
0 / 16641634 = 0.00% reads missing kmer coverage (N chars included?).
0 / 16641634 = 0.00% reads discarded as below minimum coverage threshold=1
CMD finished (25 seconds)
CMD: touch pairs.K25.stats.C50.pctSD10000.accs.ok
CMD finished (0 seconds)
CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq.ok
CMD finished (0 seconds)
CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq.ok
CMD finished (0 seconds)
CMD: ln -sf /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq left.norm.fq
CMD finished (0 seconds)
CMD: ln -sf /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq right.norm.fq
CMD finished (0 seconds)
-removing tmp dir /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/tmp_normalized_reads
Normalization complete. See outputs:
/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_1_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq
/home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/AIG_AAAOSW_8_2_C1CBGACXX.IND11_noribo_clean.fastq.normalized_K25_maxC50_minC1_pctSD10000.fq
Monday, July 9, 2018: 10:51:26 CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/normalization.ok
Converting input files. (in parallel)Monday, July 9, 2018: 10:51:26 CMD: cat /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/left.norm.fq | seqtk-trinity seq -A - >> left.fa
Monday, July 9, 2018: 10:51:26 CMD: cat /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/insilico_read_normalization/right.norm.fq | seqtk-trinity seq -A - >> right.fa
Monday, July 9, 2018: 10:51:30 CMD: touch left.fa.ok
Monday, July 9, 2018: 10:51:30 CMD: touch right.fa.ok
Monday, July 9, 2018: 10:51:30 CMD: touch left.fa.ok right.fa.ok
Monday, July 9, 2018: 10:51:30 CMD: cat left.fa right.fa > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa
Monday, July 9, 2018: 10:51:32 CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa.ok
-------------------------------------------
----------- Jellyfish --------------------
-- (building a k-mer catalog from reads) --
-------------------------------------------
* Running CMD: jellyfish count -t 40 -m 25 -s 100000000 --canonical /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa
* Running CMD: jellyfish dump -L 1 mer_counts.jf > jellyfish.kmers.fa
* Running CMD: jellyfish histo -t 40 -o jellyfish.kmers.fa.histo mer_counts.jf
----------------------------------------------
--------------- Inchworm ---------------------
-- (Linear contig construction from k-mers) --
----------------------------------------------
* Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Inchworm/bin//inchworm --kmers jellyfish.kmers.fa --run_inchworm -K 25 -L 25 --monitor 1 --DS --num_threads 6 --PARALLEL_IWORM > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa.tmp
* Running CMD: mv /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa.tmp /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa
Monday, July 9, 2018: 11:00:35 CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa.finished
--------------------------------------------------------
-------------------- Chrysalis -------------------------
-- (Contig Clustering & de Bruijn Graph Construction) --
--------------------------------------------------------
inchworm_target: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa
bowite_reads_fa: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa
chrysalis_reads_fa: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa
* Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/filter_iworm_by_min_length_or_cov.pl /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa 100 10 > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/inchworm.K25.L25.DS.fa.min100
* Running CMD: /home/urbe/anaconda3/bin/bowtie2-build --threads 40 -o 3 /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/inchworm.K25.L25.DS.fa.min100 /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/inchworm.K25.L25.DS.fa.min100 1>/dev/null
* Running CMD: bash -c " set -o pipefail;/home/urbe/anaconda3/bin/bowtie2 --local -k 2 --no-unal --threads 40 -f --score-min G,20,4 -x /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/inchworm.K25.L25.DS.fa.min100 /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa | samtools view -@ 40 -F4 -Sb - | samtools sort -m 3355443200 -@ 40 -no - - > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm.bowtie.nameSorted.bam"
* Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/scaffold_iworm_contigs.pl /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm.bowtie.nameSorted.bam /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm_scaffolds.txt
* Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis/GraphFromFasta -i /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa -r /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa -min_contig_length 200 -min_glue 2 -glue_factor 0.05 -min_iso_ratio 0.05 -t 40 -k 24 -kk 48 -scaffolding /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm_scaffolds.txt > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm_cluster_welds_graph.txt
* Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis/BubbleUpClustering -i /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/inchworm.K25.L25.DS.fa -weld_graph /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/iworm_cluster_welds_graph.txt -min_contig_length 200 > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/GraphFromIwormFasta.out
* Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis/CreateIwormFastaBundle -i /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/GraphFromIwormFasta.out -o /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/bundled_iworm_contigs.fasta -min 200
* Running CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/Chrysalis/ReadsToTranscripts -i /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/both.fa -f /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/bundled_iworm_contigs.fasta -o /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/readsToComponents.out -t 40 -max_mem_reads 50000000
* Running CMD: /usr/bin/sort --parallel=40 -T . -S 250G -k 1,1n /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/readsToComponents.out > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/chrysalis/readsToComponents.out.sort
Monday, July 9, 2018: 11:17:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_0
Monday, July 9, 2018: 11:17:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_1
Monday, July 9, 2018: 11:17:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_2
Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_3
Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_4
Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_5
Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_6
Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_7
Monday, July 9, 2018: 11:17:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_8
Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_9
Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_10
Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_11
Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_12
Monday, July 9, 2018: 11:17:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_13
Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_14
Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_15
Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_16
Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_17
Monday, July 9, 2018: 11:17:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_18
Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_19
Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_20
Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_21
Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_22
Monday, July 9, 2018: 11:17:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_23
Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_24
Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_25
Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_26
Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_27
Monday, July 9, 2018: 11:17:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_28
Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_29
Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_30
Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_31
Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_32
Monday, July 9, 2018: 11:17:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_33
Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_34
Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_35
Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_36
Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_37
Monday, July 9, 2018: 11:17:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_38
Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_39
Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_40
Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_41
Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_42
Monday, July 9, 2018: 11:17:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_43
Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_44
Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_45
Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_46
Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_47
Monday, July 9, 2018: 11:17:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_48
Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_49
Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_50
Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_51
Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_52
Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_53
Monday, July 9, 2018: 11:17:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_54
Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_55
Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_56
Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_57
Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_58
Monday, July 9, 2018: 11:17:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_59
Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_60
Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_61
Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_62
Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_63
Monday, July 9, 2018: 11:17:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_64
Monday, July 9, 2018: 11:17:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_65
Monday, July 9, 2018: 11:17:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_66
Monday, July 9, 2018: 11:17:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_67
Monday, July 9, 2018: 11:17:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_68
Monday, July 9, 2018: 11:17:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_69
Monday, July 9, 2018: 11:17:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_70
Monday, July 9, 2018: 11:17:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_71
Monday, July 9, 2018: 11:17:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_72
Monday, July 9, 2018: 11:17:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_73
Monday, July 9, 2018: 11:17:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_74
Monday, July 9, 2018: 11:17:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_75
Monday, July 9, 2018: 11:17:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_76
Monday, July 9, 2018: 11:17:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_77
Monday, July 9, 2018: 11:17:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_78
Monday, July 9, 2018: 11:17:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_79
Monday, July 9, 2018: 11:17:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_80
Monday, July 9, 2018: 11:17:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_81
Monday, July 9, 2018: 11:17:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_82
Monday, July 9, 2018: 11:17:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_83
Monday, July 9, 2018: 11:17:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_84
Monday, July 9, 2018: 11:17:50 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_85
Monday, July 9, 2018: 11:17:50 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_86
Monday, July 9, 2018: 11:17:50 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_87
Monday, July 9, 2018: 11:17:50 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_88
Monday, July 9, 2018: 11:17:51 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_89
Monday, July 9, 2018: 11:17:51 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_90
Monday, July 9, 2018: 11:17:51 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_91
Monday, July 9, 2018: 11:17:51 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_92
Monday, July 9, 2018: 11:17:52 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_93
Monday, July 9, 2018: 11:17:52 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_94
Monday, July 9, 2018: 11:17:52 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_95
Monday, July 9, 2018: 11:17:53 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_96
Monday, July 9, 2018: 11:17:53 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_97
Monday, July 9, 2018: 11:17:53 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_98
Monday, July 9, 2018: 11:17:53 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_99
Monday, July 9, 2018: 11:17:53 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_100
Monday, July 9, 2018: 11:17:54 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_101
Monday, July 9, 2018: 11:17:54 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_102
Monday, July 9, 2018: 11:17:54 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_103
Monday, July 9, 2018: 11:17:54 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_104
Monday, July 9, 2018: 11:17:54 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_105
Monday, July 9, 2018: 11:17:55 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_106
Monday, July 9, 2018: 11:17:55 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_107
Monday, July 9, 2018: 11:17:55 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_108
Monday, July 9, 2018: 11:17:56 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_109
Monday, July 9, 2018: 11:17:56 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_110
Monday, July 9, 2018: 11:17:56 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_111
Monday, July 9, 2018: 11:17:56 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_112
Monday, July 9, 2018: 11:17:57 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_113
Monday, July 9, 2018: 11:17:57 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_114
Monday, July 9, 2018: 11:17:57 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_115
Monday, July 9, 2018: 11:17:58 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_116
Monday, July 9, 2018: 11:17:58 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_117
Monday, July 9, 2018: 11:17:58 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_118
Monday, July 9, 2018: 11:17:59 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_119
Monday, July 9, 2018: 11:17:59 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_120
Monday, July 9, 2018: 11:17:59 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_121
Monday, July 9, 2018: 11:18:00 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_122
Monday, July 9, 2018: 11:18:00 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_123
Monday, July 9, 2018: 11:18:00 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_124
Monday, July 9, 2018: 11:18:01 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_125
Monday, July 9, 2018: 11:18:01 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_126
Monday, July 9, 2018: 11:18:01 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_127
Monday, July 9, 2018: 11:18:02 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_128
Monday, July 9, 2018: 11:18:02 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_129
Monday, July 9, 2018: 11:18:03 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_130
Monday, July 9, 2018: 11:18:03 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_131
Monday, July 9, 2018: 11:18:04 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_132
Monday, July 9, 2018: 11:18:04 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_133
Monday, July 9, 2018: 11:18:05 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_134
Monday, July 9, 2018: 11:18:05 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_135
Monday, July 9, 2018: 11:18:06 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_136
Monday, July 9, 2018: 11:18:06 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_137
Monday, July 9, 2018: 11:18:07 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_138
Monday, July 9, 2018: 11:18:07 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_139
Monday, July 9, 2018: 11:18:08 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_140
Monday, July 9, 2018: 11:18:08 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_141
Monday, July 9, 2018: 11:18:09 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_142
Monday, July 9, 2018: 11:18:09 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_143
Monday, July 9, 2018: 11:18:10 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_144
Monday, July 9, 2018: 11:18:10 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_145
Monday, July 9, 2018: 11:18:11 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_146
Monday, July 9, 2018: 11:18:12 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_147
Monday, July 9, 2018: 11:18:12 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_148
Monday, July 9, 2018: 11:18:13 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_149
Monday, July 9, 2018: 11:18:14 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_150
Monday, July 9, 2018: 11:18:14 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_151
Monday, July 9, 2018: 11:18:15 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_152
Monday, July 9, 2018: 11:18:16 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_153
Monday, July 9, 2018: 11:18:17 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_154
Monday, July 9, 2018: 11:18:17 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_155
Monday, July 9, 2018: 11:18:18 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_156
Monday, July 9, 2018: 11:18:19 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_157
Monday, July 9, 2018: 11:18:20 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_158
Monday, July 9, 2018: 11:18:20 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_159
Monday, July 9, 2018: 11:18:21 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_160
Monday, July 9, 2018: 11:18:22 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_161
Monday, July 9, 2018: 11:18:22 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_162
Monday, July 9, 2018: 11:18:22 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_163
Monday, July 9, 2018: 11:18:22 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_164
Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_165
Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_166
Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_167
Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_168
Monday, July 9, 2018: 11:18:23 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_169
Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_170
Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_171
Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_172
Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_173
Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_174
Monday, July 9, 2018: 11:18:24 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_175
Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_176
Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_177
Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_178
Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_179
Monday, July 9, 2018: 11:18:25 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_180
Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_181
Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_182
Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_183
Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_184
Monday, July 9, 2018: 11:18:26 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_185
Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_186
Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_187
Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_188
Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_189
Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_190
Monday, July 9, 2018: 11:18:27 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_191
Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_192
Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_193
Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_194
Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_195
Monday, July 9, 2018: 11:18:28 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_196
Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_197
Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_198
Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_199
Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_200
Monday, July 9, 2018: 11:18:29 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_201
Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_202
Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_203
Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_204
Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_205
Monday, July 9, 2018: 11:18:30 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_206
Monday, July 9, 2018: 11:18:31 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_207
Monday, July 9, 2018: 11:18:31 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_208
Monday, July 9, 2018: 11:18:31 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_209
Monday, July 9, 2018: 11:18:31 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_210
Monday, July 9, 2018: 11:18:31 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_211
Monday, July 9, 2018: 11:18:31 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_212
Monday, July 9, 2018: 11:18:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_213
Monday, July 9, 2018: 11:18:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_214
Monday, July 9, 2018: 11:18:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_215
Monday, July 9, 2018: 11:18:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_216
Monday, July 9, 2018: 11:18:32 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_217
Monday, July 9, 2018: 11:18:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_218
Monday, July 9, 2018: 11:18:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_219
Monday, July 9, 2018: 11:18:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_220
Monday, July 9, 2018: 11:18:33 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_221
Monday, July 9, 2018: 11:18:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_222
Monday, July 9, 2018: 11:18:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_223
Monday, July 9, 2018: 11:18:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_224
Monday, July 9, 2018: 11:18:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_225
Monday, July 9, 2018: 11:18:34 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_226
Monday, July 9, 2018: 11:18:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_227
Monday, July 9, 2018: 11:18:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_228
Monday, July 9, 2018: 11:18:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_229
Monday, July 9, 2018: 11:18:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_230
Monday, July 9, 2018: 11:18:35 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_231
Monday, July 9, 2018: 11:18:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_232
Monday, July 9, 2018: 11:18:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_233
Monday, July 9, 2018: 11:18:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_234
Monday, July 9, 2018: 11:18:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_235
Monday, July 9, 2018: 11:18:36 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_236
Monday, July 9, 2018: 11:18:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_237
Monday, July 9, 2018: 11:18:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_238
Monday, July 9, 2018: 11:18:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_239
Monday, July 9, 2018: 11:18:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_240
Monday, July 9, 2018: 11:18:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_241
Monday, July 9, 2018: 11:18:37 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_242
Monday, July 9, 2018: 11:18:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_243
Monday, July 9, 2018: 11:18:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_244
Monday, July 9, 2018: 11:18:38 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_245
Monday, July 9, 2018: 11:18:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_246
Monday, July 9, 2018: 11:18:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_247
Monday, July 9, 2018: 11:18:39 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_248
Monday, July 9, 2018: 11:18:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_249
Monday, July 9, 2018: 11:18:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_250
Monday, July 9, 2018: 11:18:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_251
Monday, July 9, 2018: 11:18:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_252
Monday, July 9, 2018: 11:18:40 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_253
Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_254
Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_255
Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_256
Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_257
Monday, July 9, 2018: 11:18:41 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_258
Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_259
Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_260
Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_261
Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_262
Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_263
Monday, July 9, 2018: 11:18:42 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_264
Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_265
Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_266
Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_267
Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_268
Monday, July 9, 2018: 11:18:43 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_269
Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_270
Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_271
Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_272
Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_273
Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_274
Monday, July 9, 2018: 11:18:44 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_275
Monday, July 9, 2018: 11:18:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_276
Monday, July 9, 2018: 11:18:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_277
Monday, July 9, 2018: 11:18:45 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_278
Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_279
Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_280
Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_281
Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_282
Monday, July 9, 2018: 11:18:46 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_283
Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_284
Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_285
Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_286
Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_287
Monday, July 9, 2018: 11:18:47 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_288
Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_289
Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_290
Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_291
Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_292
Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_293
Monday, July 9, 2018: 11:18:48 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_294
Monday, July 9, 2018: 11:18:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_295
Monday, July 9, 2018: 11:18:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_296
Monday, July 9, 2018: 11:18:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_297
Monday, July 9, 2018: 11:18:49 CMD: mkdir -p /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/Fb_0/CBin_298
Monday, July 9, 2018: 11:18:49 CMD: touch /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/partitioned_reads.files.list.ok
Monday, July 9, 2018: 11:18:49 CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/write_partitioned_trinity_cmds.pl --reads_list_file /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/partitioned_reads.files.list --CPU 1 --max_memory 1G --run_as_paired --seqType fa --trinity_complete --full_cleanup > recursive_trinity.cmds
Monday, July 9, 2018: 11:18:50 CMD: touch recursive_trinity.cmds.ok
Monday, July 9, 2018: 11:18:50 CMD: touch recursive_trinity.cmds.ok
--------------------------------------------------------------------------------
------------ Trinity Phase 2: Assembling Clusters of Reads ---------------------
--------------------------------------------------------------------------------
Monday, July 9, 2018: 11:18:50 CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity-plugins/BIN/ParaFly -c recursive_trinity.cmds -CPU 40 -v -shuffle
Number of Commands: 29867
succeeded(29867) 100% completed.
All commands completed successfully. :-)
** Harvesting all assembled transcripts into a single multi-fasta file...
Monday, July 9, 2018: 12:52:14 CMD: find /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/read_partitions/ -name '*inity.fasta' | /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/partitioned_trinity_aggregator.pl TRINITY_DN > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta.tmp
-relocating /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta.tmp to /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta
Monday, July 9, 2018: 12:52:15 CMD: mv /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta.tmp /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta
###################################################################
Trinity assemblies are written to /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta
###################################################################
Monday, July 9, 2018: 12:52:15 CMD: /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/util/support_scripts/get_Trinity_gene_to_trans_map.pl /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta > /home/urbe/Tools/trinityrnaseq-Trinity-v2.6.6/trinity_out_dir/Trinity.fasta.gene_trans_map
Comments
Once your assembly is complete, there are several analyses you will likely want to pursue to explore aspects of the biology of your organism based on your assembled transcripts and the input RNA-Seq data. Such studies might include:
Quantifying transcript and gene abundance. This is a prerequisite to many other analyses such as examining differentially expressed transcripts among your samples.
Quality checking your samples and biological replicates. Make sure the relationships among your samples and biological replicates make sense. If there are any confounding aspects to the data, such as outliers or batch effects, you'll want to catch them as early as possible and account for them in your further data explorations.
Perform differential expression analysis. Trinity provides direct support for several DE analysis methods, including edgeR, DESeq2, Limma/Voom, and ROTS.
Extract coding regions using TransDecoder and functionally annotate the transcripts using Trinotate.
If your organism has an assembled genome, consider using the Trinity transcriptome assemblies for gene structure annotation using PASA.