Alternative content
PROCESSORS=20
#Single_End_Layout:
samtools view --threads $PROCESSORS -b -F 4 in.bam > mapped.bam
samtools view --threads $PROCESSORS -b -f 4 in.bam > unmapped.bam
#Paired_End_Layout
samtools view --threads $PROCESSORS -b -f 2 in.bam > mapped.bam
samtools view --threads $PROCESSORS -b -F 2 in.bam > unmapped.bam