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Python script to create fastq file with random sequences
By Abhi 195 days ago
import random
def generate_random_sequence(length):
bases = ['A', 'C', 'G', 'T']
return ''.join(random.choice(bases) for _ in range(length))
def generate_random_quality(length):
return ''.join(chr(random.randint(33, 73)) for _ in range(length))
def generate_fastq_entry(sequence_length):
sequence = generate_random_sequence(sequence_length)
quality = generate_random_quality(sequence_length)
return f"@SEQ_ID\n{sequence}\n+\n{quality}\n"
def generate_fastq_file(num_entries, sequence_length, file_path):
with open(file_path, 'w') as f:
for _ in range(num_entries):
entry = generate_fastq_entry(sequence_length)
f.write(entry)
# Generate a FASTQ file with 5 entries, each with a sequence length of 50 bases
generate_fastq_file(100, 50, 'random.fastq')
