On linux OS you can extract human chromosome length with following command
curl -s ftp://hgdownload.cse.ucsc.edu/goldenPath/hg19/database/chromInfo.txt.gz | gunzip -c
from a Broad Institute site:
"N50 is a statistical measure of average length of a set of sequences. It is used widely in genomics, especially in reference to contig or supercontig lengths within a draft assembly.
Given a set of sequences of...
Trinity, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. Trinity combines three independent software modules:...