Archana Malhotra
Computational Biologist
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Protocol for De novo Genome Assembly using Illumina Reads
By BioStar 2028 days agoIn this protocol, we address and describe the de novo assembly method for small to medium-sized genomes.NextDenovo: string graph-based de novo assembler for TGS long reads
By Jit 2406 days agogithub.com - NextDenovo is a string graph-based de novo assembler for TGS long reads. It uses a "correct-then-assemble" strategy similar to canu, but requires significantly less computing resources and storages. After assembly, the per-base error rate...BASE: a practical de novo assembler for large genomes using long NGS reads
By Rahul Nayak 2849 days agogithub.com - new de novo assembler called BASE. It enhances the classic seed-extension approach by indexing the reads efficiently to generate adaptive seeds that have high probability to appear uniquely in the genome. Such seeds form the basis for BASE...Flye: Fast and accurate de novo assembler for single molecule sequencing reads
By BioJoker 2683 days agogithub.com - Flye is a de novo assembler for single molecule sequencing reads, such as those produced by PacBio and Oxford Nanopore Technologies. It is designed for a wide range of datasets, from small bacterial projects to large mammalian-scale assemblies. The...LoRMA: A tool for correcting sequencing errors in long reads
By Abhimanyu Singh 2891 days agogite.lirmm.fr - An error correction method that uses long reads only. The method consists of two phases: first, we use an iterative alignment-free correction method based on de Bruijn graphs with increasing length of k-mers, and second, the corrected reads are...SViper: Swipe your Structural Variants called on long (ONT/PacBio) reads with short exact...
By Neel 2420 days agogithub.com - Call sviper ~$ ./sviper -s short-reads.bam -l long-reads.bam -r ref.fa -c variants.vcf -o polished_variants This will output a polished_variants.vcf file, that contains all the refined variants. Sometimes it is helpful to look at the...Bioinformatics WalkIn at NII
ADVERTISEMENT OF WALK-IN-INTERVIEW NAME OF THE POST : Bioinformatician (Part time 3 days in a week) (One Position only) DURATION : One Year NAME OF THE PROJECT : Next generation sequencing facility EDUCATIONAL QUALIFICATIONS : At least a...Postdoctoral Fellowship in Bioinformatics at pesolelab
Job Description: Bioinformatics postdoc positions are available in the area of genomics with main focus on exome and RNAseq technologies by ultra high-throughput sequencing platforms. Successful applicants should have the following qualities: 1)...QuasR: Quantification and annotation of short reads in R
By Neel 1820 days agowww.bioconductor.org - The QuasR package (short for Quantify and annotate short reads in R) integrates the functionality of several R packages (such as IRanges (Lawrence et al. 2013) and Rsamtools) and external software (e.g. bowtie, through the Rbowtie package, and...Pilon
By Rahul Nayak 3832 days agogithub.com - Pilon is a software tool which can be used to: Automatically improve draft assemblies Find variation among strains, including large event detection Pilon requires as input a FASTA file of the genome along with one or more BAM files of reads...
