Jit
Enthusiast computational biologist with business dream !!!
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RefKA: A fast and efficient long-read genome assembly approach for large and complex genomes
By Rahul Nayak 2324 days agogithub.com - RefKA, a reference-based approach for long read genome assembly. This approach relies on breaking up a closely related reference genome into bins, aligning k-mers unique to each bin with PacBio reads, and then assembling each bin in parallel...GraphMap - A highly sensitive and accurate mapper for long, error-prone reads
By Jit 3383 days agogithub.com - GraphMap - A highly sensitive and accurate mapper for long, error-prone reads http://www.nature.com/ncomms/2016/160415/ncomms11307/full/ncomms11307.htmlFeatures Mapping position agnostic to alignment parameters. ...Scaffolding of a bacterial genome using MinION nanopore sequencing
By Rahul Agarwal 4084 days agowww.nature.com - Second generation sequencing has revolutionized genomic studies. However, most genomes contain repeated DNA elements that are longer than the read lengths achievable with typical sequencers, so the genomic order of several generated contigs cannot...Nanopore adaptor !
By Jit 2412 days agogithub.com - Porechop is a tool for finding and removing adapters from Oxford Nanopore reads. Adapters on the ends of reads are trimmed off, and when a read has an adapter in its middle, it is treated as chimeric and chopped into separate reads....
Run miniasm assembler on nanopore reads !
Last updated 3189 days ago by Jit Comments (5)Miniasm is a very fast OLC-based de novo assembler for noisy long reads. It takes all-vs-all read self-mappings (typically by minimap) as input and outputs an assembly graph in the GFA format. Different from mainstream...Interview Mark Sansom (U. Oxford): Simulations of Membrane Proteins
By Shreya Ganguli 4722 days agoWorkshop in Bioinformatics, 4/June/2012 Campus Vida's Research Centers organize in Santiago de Compostela the Workshop in Bioinformatics. This event addressed issues such as structural bioinformatics, biological modelling and mining bioinformatics...Postdoctoral Fellowship at Department of Psychiatry, Warneford Hospital, Oxford
Applications are invited for a postdoctoral research assistant to work in the Translational Neuroscience and Dementia Research Group (TNDRG) on a project using informatics approaches to understand and prevent dementia, specifically on the role of...FinisherSC: a repeat-aware and scalable tool for upgrading de novo assembly using long reads
By Jit 3483 days agokakitone.github.io - FinisherSC, a repeat-aware and scalable tool for upgrading de novo assembly using long reads. Experiments with real data suggest that FinisherSC can provide longer and higher quality contigs than existing tools while maintaining high...npScarf: Scaffolding and Completing Assemblies in Real-time Fashion
By Jit 3398 days agogithub.com - npScarf (jsa.np.npscarf) is a program that scaffolds and completes draft genomes assemblies in real-time with Oxford Nanopore sequencing. The pipeline can run on a computing cluster as well as on a laptop computer for microbial datasets. It...Hercules: a profile HMM-based hybrid error correction algorithm for long reads
By Jit 2944 days agogithub.com - Choosing whether to use second or third generation sequencing platforms can lead to trade-offs between accuracy and read length. Several studies require long and accurate reads including de novo assembly, fusion and structural variation detection....
