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GAM-NGS: genomic assemblies merger for next generation sequencing
By Jit 2746 days agogithub.com - GAM-NGS is a tool able to merge two or more assemblies in order to improve contiguity and correctness. It can be used on all NGS-based assembly projects and it shows its full potential with multi-library Illumina-based projects. With more than 20...BBTools for bioinformatician !
By Surabhi Chaudhary 2474 days ago Comments (1)BBTools can do almost everything now !Porechop: tool for finding and removing adapters from Oxford Nanopore reads
By Rahul Nayak 2371 days agogithub.com - Porechop is a tool for finding and removing adapters from Oxford Nanopore reads. Adapters on the ends of reads are trimmed off, and when a read has an adapter in its middle, it is treated as chimeric and chopped into separate reads. Porechop...AlignQC: A tool for assessing an alignment, and generating reports that are easy to share
By Jit 2302 days agowww.healthcare.uiowa.edu - Long read alignment analysis. Generate a reports on sequence alignments for mappability vs read sizes, error patterns, annotations and rarefraction curve analysis. The most basic analysis only requires a BAM file, and outputs a web browser...Understanding HiFi Reads !
By Rahul Nayak 976 days agopacb.com - While little public data is available for either of the new synthetic long read approaches, Illumina showed an example comparison earlier this year at the Festival of Genomics & Biodata conference (FoG 2022). In the IGV screenshot...1mb long DNA with Nanopore technology
By Jit 2532 days agoLongest read of 1mb achievements with NanoporePacasus: Correction of palindromes in long reads from PacBio and Nanopore
By BioStar 2204 days agogithub.com - Tool for detecting and cleaning PacBio / Nanopore long reads after whole genome amplification. Check the poster from the Revolutionizing Next-Generation Sequencing (2nd edition) conference in the source...CoLoRMap: Correcting Long Reads by Mapping short reads
By Jit 2288 days agogithub.com - Second generation sequencing technologies paved the way to an exceptional increase in the number of sequenced genomes, both prokaryotic and eukaryotic. However, short reads are difficult to assemble and often lead to highly fragmented assemblies....Rcorrector: efficient and accurate error correction for Illumina RNA-seq reads
By BioStar 1755 days agogithub.com - Rcorrector has an accuracy higher than or comparable to existing methods, including the only other method (SEECER) designed for RNA-seq reads, and is more time and memory efficient. With a 5 GB memory footprint for 100 million reads, it can be run...DADA2: Fast and accurate sample inference from amplicon data with single-nucleotide resolution
By Jit 1475 days agobenjjneb.github.io - The DADA2 tutorial goes through a typical workflow for paired end Illumina Miseq data: raw amplicon sequencing data is processed into the table of exact amplicon sequence variants (ASVs) present in each sample. The DADA2...
