2162 days ago
Installing Porechop on Ubuntu !
➜ Tools git:(master) ✗ git clone https://github.com/rrwick/Porechop.g...[--format {auto,fasta,fastq,fasta.gz,fast...default: 16) Barcode binning settings: Control the binning of read...fault: False) Adapter search settings: Control how the program det...2145 days ago
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Bash script to download SRA file !
#We can use the sratoolkit to directly pull the sequence data (in paired FASTQ format) from the archive. fastq-dump is in the SRA toolkit. I...a particular sequencing run ID fastq-dump --split-files SRR1770413...ools, so it's necessary to put things into FASTQ for further processi...1598 days ago
Bash script to alignment of short reads against reference genome !
bwa mem -t 40 -R '@RG\tID:K12\tSM:K12' \...E.coli_K12_MG1655.fa SRR1770413_1.fastq.gz SRR1770413_2.fastq.gz \ | samtools view -b...he sample name K12" #reference and FASTQs E.coli_K12_MG1655.fa SRR1770...E.coli_K12_MG1655.fa SRR341549_1.fastq.gz SRR341549_2.fastq.gz \...1598 days ago
Bash command to install GATK, Bedtools and SnpEff !
(my_GATK) [wsu29@bladeamd-2 tmp]$ conda install gatk Collecting package metadata...kshop/wsu29/anaconda3/envs/my_GATK added / updated specs:...py38_0 156 KB gatk-3.8 |...:certifi-2019.11.28-py38_0 gatk bioconda/noarch...1594 days ago