Comment on "REAPR: a universal tool for genome assembly evaluation"
Reapr is a tool trying to find explicit errors in the assembly based on incongruently mapped reads. It is heavily based on too low span coverage, or reads mapping...1388 days ago
1571 days ago
Comment on "Perl script to count number of Ns in a multifasta file !"
If you want to count all 'N' in miltifasta file. (base) ? output_2_test git:(master) ? more scaffolds.fasta | grep -Ho N * | uniq -c ...1614 days ago
Comment on "Extract fasta sequence from a multifasta file with fasta header Ids"
perl -ne 'if(/^>(\S+)/){$c=grep{/^$1$/}qw(id1 id2)}print if $c' fasta.file If you have a large number of sequences that you want to extract, then you most likely ha...1961 days ago
Comment on "HALC: High throughput algorithm for long read error correction"
Inputs Long reads in FASTA format. Contigs assembled from the corresponding short reads in FASTA format. The initial short reads in FASTA format (only...2142 days ago
2150 days ago
Comment on "Installing Porechop on Ubuntu !"
➜ MyPassport porechop -i /media/urbe/MyDDrive/ONTdata/allONT/allONT.fasta -t 40 > ONT_choppedNcorrected.fa Loading reads/media/urbe/MyDDrive/ONTdata/allONT/allONT.f...2157 days ago
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Comment on "Run miniasm assembler on nanopore reads !"
Here’s the quick and dirty of what was done: 1 Run minimap: This uses a pre-built set of defaults (the ava-pb in the code below) for analyzing PacBio...2218 days ago