Results for "BAM"

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  • Download blasr 1.3 version

    ...local/bin/samtools sort -@ 20 -no - temporarySam > FALCON-Unzip-Scaff.bamMapping long-reads using blasr...'anchor_contig_info.dat': No such file or directorymv FALCON-Unzip-Scaff.bam FALCON-Unzip-Scaff-with-repea...

    2150 days ago

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  • Extract list of read ID from bam file: samtools view mbi.sorted.bam |perl -ne '/ID/ && print' > extractedID.sam #Extract #Ids #SAM #BAM #NGS

    3634 days ago

  • http://bam.iobio.io/ #bam #vcf #analysis

    3443 days ago

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  • Genome Covered !

    zero=$(bedtools genomecov -ibam BAM -g hg38.fasta -bga | awk '$4==0 {bpCountZero+=($3-$2)} {print bpCountZero}' | tail -1) nonzero=$(bedtools genomecov -ibam BAM -g hg38.fasta -bga | awk '$4>0 {bpCountNonZero+=($3-$2)} {print bpCountNonZero}' | tail -1) percent=$(bc

    2209 days ago

  • Coverage / Depth of reads !

    # get total number of bases covered at MIN_COVERAGE_DEPTH or higher samtools mpileup mapping_result_sorted.bam | awk -v X="${MIN_COVERAGE_DEPTH}" '$4>=X' | wc -l 32876 # get le...

    2209 days ago

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  • Structure of Binary files used for storing sequencing data-bam and sff

    Many times bioinformatician needs to parse binary files like bam and sff. Advantage of binary files is that they occupy less space in memory with maximum information content. Link for those who looking for structure of Bam and sff file: Bam: http://samtools.sourceforge.net/SAMv1.pdf (from...

    Tags: bam, sff, sam, iontorrent data, 454 data, pyrosequencing data, hxd, hexadecimal, binary files, sequencing

    3919 days ago

  • sam to bam conversion !!

    To do sam to bam conversion, follow the following commands :-  Code: $ samtools view -b -S file.sam > file.bam Then you will need to use  Code: $ samtools sort file.bam file-sorted followed by Code: $ samtools index file-sorted.bam in order to get an indexed fil...

    Tags: sam, bam, conversion

    2290 days ago

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