Results for "FASTQ"
Blogs
BBTools for bioinformatician !
...h means N (in either fasta or fastq format). The BBMap package au...mat.sh in=reads.sam out=reads.fastq Verify pairing and optio...0 adderrors=false out=perfect.fastq reads=1000 minlength=18 maxle...omma-delimited (e.g. in=file1.fastq,file2.fastq,file3.fastq). And...2263 days ago
- ...have paired reads and # you expect high gene density with UTR # overlap (use FASTQ input file format #...
2125 days ago
Converting a VCF into a FASTA given some reference !
...by others to convert InDels as well, e.g. this by David Eccles ./vcf2fq.pl -f <input.fasta> <all-site.vcf> > <output.fastq> https://github.com/gring...2108 days ago
CANU genome assembly parameters !
...Grid=false \ [other-options] \ read_file.fastq.gz A default Canu&nbs...Threads=2 useGrid=false \ -pacbio-raw \ ~/pacbio/dna/sample_reads.fastq.gz There is a brilliant ...1937 days ago
Protocol for De novo Genome Assembly using Illumina Reads
...pathogens study can lead to treatments for infectious diseases. Raw NGS dataReads can be saved as a Fasta file as text or in a FastQ file with their attributes. FastQ is the most common read file...1196 days ago
Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant calling Read alignment (Bowtie 2) Sort and index...869 days ago
Useful Bioinformatics Analysis Tools !
... DSRC 2: Industry-oriented compression of FASTQ files, Bioinformatics,&n...ski, Sz., Compression of DNA sequences in FASTQ format, Bioinformatics,&...QSqueezer Experimental high-end compressor of FASTQ files. • Deo...855 days ago
Common steps for reads mapping !
...have their own indexing tools. Prepare the read data: The reads should be in a format that is compatible with the mapper. Most mappers accept FASTQ or BAM files. Depending on th...415 days ago
