BBTools for bioinformatician !
...ly some Illumina software versions produces odd things like a handful of Q0 called bases or Ns with Q>0, neither of which make any sense in the Phred scale. Sampling reads &...2279 days ago
Genome Assembly Tools and Software - PART2 !!
...e data from automated DNA sequencers prior to sequence assembly and other downstream uses. The quality trimming portion of lucy makes use of phred quality scores, such as those...2694 days ago
2877 days ago
Genome Assembly Tools and Software - PART1 !!
...nose, report, and automatically improve de novo genome assemblies. Phred/Phrap/Consed 29.0 – DNA...tection, available for Windows and Mac OS X. Aligner is compatible with Phred-Phrap and fully supports sequ...2694 days ago
Estimate Genome Size with Jellyfish and R
...y allocation #-o -output=string Output file name #--min-quality-char Base quality value. Version 2.2.3 of Jellyfish uses the “Phred” score, where "?" = 30 jel...2254 days ago
A quick guide to Phred scaling
A quick guide to Phred scaling• Phred value = −10 * log10(ε)• Examples:• 90% confidence (10% error rate) = Q10• 99% confidence (1% error rate) = Q20• 99.9% confidence (.1% error rate) = Q30• SAM encoding adds 33 to the value (becauseASCII 33 is the firs...Tags: quick, guide, Phred, scaling
1542 days ago
Comment on "A quick guide to Phred scaling"
Phred scaling is a widely used syste...read. Here is a quick guide to Phred scaling: Phred quality scores: Phred quality scores are represente...%. Logarithmic scale: The Phred scale is logarithmic, which m...he character code equal to the Phred score plus 33. For example, a...423 days ago