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  • NGS Glossary !!

    ...the sample of interest. Genotype likelihoods are usually expressed as a Phred-scaled probability, where P =...ful for identifying structural rearrangements, including gene fusions. Phred-scaled probability: a scaled...

    2877 days ago

  • Genome Assembly Tools and Software - PART1 !!

    ...nose, report, and automatically improve de novo genome assemblies. Phred/Phrap/Consed 29.0 – DNA...tection, available for Windows and Mac OS X. Aligner is compatible with Phred-Phrap and fully supports sequ...

    2694 days ago

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  • #Sickle ( https://github.com/najoshi/sickle ) to #trim #reads using a 20-Phred quality threshold

    2499 days ago

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  • A quick guide to Phred scaling

    A quick guide to Phred scaling• Phred value = −10 * log10(ε)• Examples:• 90% confidence (10% error rate) = Q10• 99% confidence (1% error rate) = Q20• 99.9% confidence (.1% error rate) = Q30• SAM encoding adds 33 to the value (becauseASCII 33 is the firs...

    Tags: quick, guide, Phred, scaling

    1542 days ago

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  • Comment on "A quick guide to Phred scaling"

    Phred scaling is a widely used syste...read. Here is a quick guide to Phred scaling: Phred quality scores: Phred quality scores are represente...%. Logarithmic scale: The Phred scale is logarithmic, which m...he character code equal to the Phred score plus 33. For example, a...

    423 days ago