Comment on "Short-read assembly using Spades !"
...of high quality and do not contain sequencing errors or low-quality bases. This step can be performed using various tools such as Trimmomatic or FastQC. Running SPAdes: After q...412 days ago
Comment on "Running Trinity on RNA-seq !"
Once your assembly is complete, there are several analyses you will likely want to pursue to explore aspects of the biology of your organism based on your assembled t...2119 days ago
2134 days ago
Comment on "Basic command-line to run BLAST"
...gaAln2_mouse.tab -evalue 1e-20 -outfmt '6 qseqid qlen qstart qend sseqid slen sstart send pident gaps evalue length frames qcovs bitscore' -max_target_seqs 1 -num_threads 40 -qcov_hsp_perc 90 -threshold 90 -...2241 days ago
Comment on "BLAST+ updated !!!"
..._file] [-out output_file] [-evalue evalue] [-word_size int_value] [-gapopen open_penalty] [-gapextend extend_penalty] [-perc_identity float_value] [-qcov_hsp_perc float_value] [-max...2513 days ago
2928 days ago