RNA-Seq Data Pathway and Gene-set Analysis Workflows
It describe the GAGE (Luo et al., 2009) /Pahview (Luo and Brouwer, 2013) workflows on RNA-Seq data pathway analysis and gene-set analysis. The gage package (2.12.0) now includes a new tutorial, “RNA-Seq Data Pathway and Gene-set Analysis Workflows“. First cover a full workf...Tags: R, Bioconductor, RNA-seq, GAGE, Pathway
3832 days ago
Surrogate Variable Analysis (SVA)
The sva package contains functions for removing batch effects and other unwanted variation in high-throughput experiment. Specifically, the sva package contains functions for the identifying and building surrogate variables for high-dimensional data sets. Surrogate variables are covariates constr...Tags: RNA-seq, Genomics, R, Bioconductor
3474 days ago
Webinar on 'An integrated RNA and DNA approach to unravel genetic regulation in cancer'
Webinar on 'An integrated RNA and DNA approach to unravel genetic regulation in cancer' Abstract Whole exome DNA sequencing (WES) or whole genome DNA sequencing (WGS) allows detection of mutations and polymorphisms in all exonic and genomic regions, respectively, while messenger RNA sequencin...Tags: NGS, data analysis, genomics, transcriptomics, DNA-seq, RNA-seq
3370 days ago
RNA-Seq De novo Assembly Using Trinity
Trinity, developed at the Broad Institute and the Hebrew University of Jerusalem, represents a novel method for the efficient and robust de novo reconstruction of transcriptomes from RNA-seq data. Trinity combines three independent software modules: Inchworm, Chrysalis, and Butterfly, applied seq...Tags: Bioinformatics, NGS, RNA-seq, Assembly, Trinity, Illumina
2964 days ago
Scallop: reference-based transcriptome assembler for RNA-seq
Scallop is an accurate reference-based transcript assembler. Scallop features its high accuracy in assembling multi-exon transcripts as well as lowly expressed transcripts. Scallop achieves this improvement through a novel algorithm that can be proved preserving all phasing paths from reads and p...Tags: Scallop, reference-based, transcriptome, assembler, RNA-seq, Tools
2188 days ago
Tags: Installing, Trinity, RNA-seq, NGS, Assembly, Transcriptomics
2132 days ago
RNA-seq Analysis Workshop Course Materials
RNAseq can be roughly divided into two "types": Reference genome-based - an assembled genome exists for a species for which an RNAseq experiment is performed. It allows reads to be aligned against the reference genome and significantly improves our ability to reconstruct transcripts. This cate...Tags: RNA-seq, Analysis, Workshop, Course, Materials, NGS
2132 days ago
Tags: NCBI, Magic-BLAST, Documentation, RNA-seq, Mapping, BLAST
2089 days ago
BioJupies: Automatically Generates RNA-seq Data Analysis Notebooks
With BioJupies you can produce in seconds a customized, reusable, and interactive report from your own raw or processed RNA-seq data through a simple user interface BioJupies now supports user accounts! Sign in from the top right corner of the page for access to unlimited private notebooks, RNA-...Tags: BioJupies, Automatically, Generates, RNA-seq, Data, Analysis, Notebooks
1231 days ago
Rcorrector: efficient and accurate error correction for Illumina RNA-seq reads
Rcorrector has an accuracy higher than or comparable to existing methods, including the only other method (SEECER) designed for RNA-seq reads, and is more time and memory efficient. With a 5 GB memory footprint for 100 million reads, it can be run on virtually any desktop or server. The software ...Tags: Rcorrector, efficient, accurate, error, correction, Illumina, RNA-seq, reads
1550 days ago