Results for "discordant read pairs"

Blogs

  • Understanding reads mapping and flags !

    Linear Alignment: An alignment of a read to a single reference sequence that...ic Alignment: An alignment of a read that cannot be represented as...plementary alignment flag. Chimeric reads are indicative of structural...d the representative alignment. One read...

    1851 days ago

  • AWK for beginners !

    AWK is a standard tool on every POSIX-compliant UNIX system. It&...Why use AWK instead of Perl? Readability. AWK is easier to read than Perl. For simple text-pr...# BEFORE any of the files are read. The END pattern is true afte...localvar) { # You've already seen print print "Hello...

    1850 days ago

  • Bioinformatics tools for telomere to telomere assembly !

    ● Merfin – k-mer-based assembly and variant calling evalu...-mer count information from raw, short-read sequencing data to genotype a...ipeline for the classification of long-read transcripts that can assess t...-quality consensus sequences from HiFi reads...

    1006 days ago

  • Protocol for De novo Genome Assembly using Illumina Reads

    In this protocol, we address and describ...equencing system used, these "reads" range from 20 to 1000 nucleotide base pairs (bp) in length. Usually, len...ctious diseases. Raw NGS dataReads can be saved as a Fasta file...A significant range of short-read assemblers are available. Ever...

    1219 days ago

  • Run bash script in Perl program !

    BioPerl is a compilation of Perl modules that can be used to build bioinformatics-rela...can also be used with or without the shell. You can read about it more in the document...d to Perl script: For further information, you can read...

    651 days ago

  • Innovative blog and portfolio website!

    Do you ever feel overwhelmed or frightened by browsing through someone else's beautiful, responsive portfolio website links and wishing you could create one for yourself?So there you have it!Read...

    1062 days ago

  • Illumina based assembly pipeline steps !

    Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant calling...(iVar; amplicon data only) Duplicate read marking (picard; optional) A...Present QC and visualisation for raw read,...

    891 days ago

  • Useful Bioinformatics Analysis Tools !

    CoMeta Classificier of reads from metagenomic sequencing experiments. &...h;234, DSRC Compressor of sequencing reads. •  Roguski,...Experimental compressor of sequencing reads. •  Grabowsk...NE, 2014; 9(2):e88901, RECKONER Read...

    878 days ago

  • List of comparative genomics resources !

    3D-GENOMICS -- A Database to Compare Structural and Functional Annotations of Prot...conserved non-coding blocks from upstream regions of putative orthologous gene pairs from man, mouse, rat, fugu, M...

    691 days ago

  • Online resources on must-read papers in evolutionary biology, for a literature club

    1. *Nick Barton:* - The textbook "Evolution" by Nick Barton, with resources f...ig et al. 2013. Genetic incompatibilities are widespread within species. Nature. 2...link to 'papers every evolutionary biologist should read'...

    691 days ago