How to separate FASTA sequences by their name in files?
I have one big file with lots of fasta sequences. I would like to separate the sequences by their name in files. Can anyone please help me to do so. Thanks for your time. Sample fasta sequences in file: >SBI_03055_PP_HisKa|Streptomyces bingchenggensis BCW-1MSSSDAARTGSAEGARDSKRVRTRRTLRDWIVDVC...Tags: FASTA, Sequences, Files, Perl
3936 days ago
Replace N characters with random nucleotide in a FASTA file.
During sequencing and assembly process Fasta files typically contain invalid characters such as "NNNN", how can I remove those with randon nucleotide charaters A T G C.Tags: FASTA, Perl, N characters, Random
3882 days ago
Tags: Protein, Sift, Error, FASTA, Server
3006 days ago
Converting a VCF into a FASTA given some reference !
Samtools/BCFtools (Heng Li) provides a Perl script vcfutils.pl which does this, the function vcf2fq (lines 469-528) This script has been modified by others to convert InDels as well, e.g. this by David Eccles ./vcf2fq.pl -f <input.fasta> <all-site.vcf> ...Tags: Convert, VCF, FASTA, reference, genome, assembly, phase
2130 days ago
UniqueKmer: Generate unique KMERs for every contig in a FASTA file
Generate unique k-mers for every contig in a FASTA file. Unique k-mer is consisted of k-mer keys (i.e. ATCGATCCTTAAGG) that are only presented in one contig, but not presented in any other contigs (for both forward and reverse strands). This tool accepts the input of a FASTA file consisting of ...Tags: Unique, Kmer, Mers, Contigs, FASTA, Genome
884 days ago
Download mutliple fasta file from NCBI in one GO!!
if you have less time, then use three ways mentioned in bookmark link to extract/download all fasta sequences in single click given that you already have a list of GIs or accession IDs . Alternatively, use one liner perl script: perl -ne 'if(/^>(\S+)/){$c=$i{$1}}$c?print:chomp;$i{$_}=1 if @A...Tags: fasta, multiple fasta, ncbi, entrez, perl
3924 days ago