2183 days ago
Gap filling or Contigs extensions tools !
There are many tools to perform gap filling using Illumina short reads, fo...aired reads" or "Toward almost closed genomes with GapFiller". There are a...take a look at the IMAGE tool: http://genomebiology.com/2010/11/4/R41...n't interested in assembling the full genome,...2158 days ago
Frequent Paired-end reads (PE 2x100) mapping command lines
bowtie2 -x hs37m -X 650 -q -1 r1.fq -2 r2.fq -S r12.bowtie2.sam bwa aln hs37m.fa r1.fq > r1.sai && bwa aln hs37m.fa r2.fq > r2.sai \ &&...2175 days ago
Understanding BLASTn output format 6 !
BLASTn output format 6 BLASTn maps DNA against DNA, for example gene sequences against a reference genomeblastn -query genes.ffn -subject genome.fna -outfmt 6 BLASTn tabula... 2. sseqid subject (e.g., reference genome)...2132 days ago
List of non-commercial NGS genotype-calling software
Meaningful analysis of next-generation sequencing (NGS) data, which are produced extens...tp://www.broadinstitute.org/gsa/wiki/index.php/The_Genome_Analysis_Toolkit Multi-sample...r.ac.uk/resources/software/margarita) MaCH http://genome....2089 days ago
Parallel Processing with Perl !
Here is a small tutorial on how to make best use of multiple processors for bioinformatics analysis. One best way is using perl threads and forks. Knowing how these thr...2073 days ago
Frequent parameters for bioinformatics tools !
Third party executable parameters and options. &n...confidence: 40 falcon_ns.task_options.HGAP_GenomeLength_str: 6000000 Pbcoreto...ABR) --input_type contig bowtie2 (mapping) --sensitive minimap2 (mapping) -a -x map-ont sam...1279 days ago
Bioinformatics in Africa:- Part 1
The Institut Pasteur de Côte d’Ivoire was created by the N°72511 l...training in bioinformatics and genome data analysis. Sho...7 a yearly regional course of initiation and using genome...1183 days ago
Bioinformatics tools for genome assembly !
There are numerous genome assembly tools available, each with its...is a list of some widely used genome assembly tools as of my last...cell and multi-cell bacterial genomes, as well as small eukaryotic...assembling large and complex genomes. MaSuRCA: A hybrid asse...279 days ago
Steps to find all the repeats in the genome !
To find repeats in a genome from 2 to 9 length using a Perl script, you can use...he RepeatMasker website[0]. Prepare the genome sequence: Make sure you have the genome sequence in a FASTA file form...ecific needs and the characteristics of your genome....241 days ago