Results for "split reads"
Blogs
Understating pacbio reads name !
m140415_143853_42175_c100635972550000001823121909121417_s1_p0/553/3100_11230 0.99 24...ole number Subread Region (start_stop using polymerase read coordinates) readScore barcodeScore...1993 days ago
CANU genome assembly parameters !
...se two cores (parameter -maxThreads=2) and you would like to disa...ze=[g|m|k] \ -maxThreads=2 \ useGrid=f...genomeSize=2m \ -maxThreads=2 useGrid=false \ -pacb...quences after correction, trim and split based on consensus evidence....1948 days ago
Understanding reads mapping and flags !
...the supplementary alignment flag. Chimeric reads are indicative of structural...c genes in RNA-seq. In short, chimeric reads can be split in to two or more parts, each...at GATK can realignment those representative reads to the correctly position via...1840 days ago
- ...file supplied. Each record is split by the FS delimiter, # which...ay with the built-in function split() n = split("foo:bar:b...the fourth character # Split on a delimiter n = split...=> prints foobar # Reads a line from standard input an...
1839 days ago
Perl one-liner for beginners !
I often use the following arguments to perl: -e Makes the line of code be executed i...lWordsOnlyLowercase.txt Go through file, split line at each space and print...one per line. perl -ne 'print join("\n", split...1384 days ago
Bioinformatics tools for telomere to telomere assembly !
● Merfin – k-mer-based assembly and varia...generate high-quality consensus sequences from HiFi reads (Zev Kronenberg @zevkron...h; tool for splice alignment of long transcriptomic reads to a genome, guided by a data...995 days ago
Protocol for De novo Genome Assembly using Illumina Reads
...The DNA of the target organism is split up into millions of tiny part...the sequencing system used, these "reads" range from 20 to 1000 nucleo...rs (bp) in length. Usually, length reads of 36 - 150 bp are produced f...infectious diseases. Raw NGS dataReads can be saved as a Fasta file...1208 days ago
Illumina based assembly pipeline steps !
Illumina Merge re-sequenced FastQ files (cat) Read QC (FastQC) Adapter trimming (fastp) Removal of host reads (Kraken 2; optional) Variant...880 days ago
Useful Bioinformatics Analysis Tools !
CoMeta Classificier of reads from metagenomic sequencing experiments. &bull...):22–234, DSRC Compressor of sequencing reads. • Roguski,..., ORCOM Experimental compressor of sequencing reads. • Grabowski...867 days ago
Common steps for reads mapping !
Mapping reads to a reference genome is an essent...eneral steps to follow for mapping reads to a genome: Choose a re...e reference genome: Before mapping reads, the reference genome needs t...mapping rate, the number of mapped reads, and the mapping quality scor...427 days ago
