Thanks for all useful links. I found this job website also very useful https://www.timeshighereducation.com/unijobs/searchjobs/?Keywords=bioinformatics&radialtown=&LocationId=&RadialLocation=5
Reads simulator
Wgsim is a small tool for simulating sequence reads from a reference genome. It is able to simulate diploid genomes with SNPs and insertion/deletion (INDEL) polymorphisms, and simulate reads with uniform substitution sequencing...
SAMtools: Primer / Tutorial by Ethan Cerami, Ph.D.keywords: samtools, next-gen, next-generation, sequencing, bowtie, sam, bam, primer, tutorial, how-to, introductionRevisions 1.0: May 30, 2013: First public release on...
The purpose of this tutorial is to introduce students to the frequently used tools for NGS analysis as well as giving experience in writing one-liners. Copy the required files to your current directory, change directory (cd) to the linuxTutorial...
Linux Commands Cheat Sheet File System ls — list items in current directory ls -l — list items in current directory and show in long format to see perimissions, size, an modification...
Horizontal gene transfer (HGT), the transfer of genetic material between organisms, is crucial for genetic innovation and the evolution of genome architecture. Existing HGT detection algorithms rely on a strong phylogenetic signal distinguishing the...
DarkHorse is a bioinformatic method for rapid, automated identification and ranking of phylogenetically atypical proteins on a genome-wide basis. It works by selecting potential ortholog matches from a reference database of amino acid...
Run blastn -help then look for the field called outfmt
*** Formatting options
-outfmt <String>
alignment view options:
0 = pairwise,
1 = query-anchored showing identities,
2 = query-anchored no identities,
3 =...
I am doing megablast of a contig against NR database, but unable to understand this
-outfmt '6 qseqid staxids qstart qend sstart send qseq sseq evalue length'
Can you please help me to undestand all these.